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Brain (Mouse) Membrane Lysate - normal tissue (ab30149)

Q&A (1)Specific References (1)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.15% Beta mercaptoethanol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab30149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Brain (Mouse) Membrane Lysate - normal tissue (ab30149)

This product has been referenced in:
  • Fink C  et al. Claudin-11 is over-expressed and dislocated from the blood-testis barrier in Sertoli cells associated with testicular intraepithelial neoplasia in men. Histochem Cell Biol 131:755-64 (2009). WB . Read more (PubMed: 19241088) »

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Publishing research using ab30149 ? Please let us know so that we can cite the reference in this datasheet

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looking for these products in mice 6 weeks or older, are these lysates from adults? or are you able to produce this?

Thank you for contacting us. These two products are supplied as adult lysates, all older than 6 weeks , so they should be suitable for your studies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"