Overview
- Product nameAnti-GRASP65 antibodySee all GRASP65 primary antibodies ...
- DescriptionRabbit polyclonal to GRASP65
- Tested applicationsIP, WB, ICC/IF, IHC-P, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
- Immunogen
Recombinant full length 6-his tagged protein (Mouse)
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab30315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/5000 - 1/10000. Predicted molecular weight: 46 kDa. |
| ICC/IF | ICC/IF: 1/5000. |
| IHC-P | IHC-P: 1/5000. |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent concentration. PubMed: 22355340 |
Target
- FunctionStacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
- Sequence similaritiesBelongs to the GORASP family.
Contains 1 PDZ (DHR) domain. - Post-translational
modificationsPhosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis. - Cellular localizationGolgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
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Database links
- Entrez Gene: 64689 Human
- Entrez Gene: 64689 Human
- Entrez Gene: 74498 Mouse
- Entrez Gene: 56082 Rat
- Omim: 606867 Human
- SwissProt: Q3SYG7 Human
- SwissProt: Q9BQQ3 Human
- SwissProt: Q9BQQ3 Human
- SwissProt: Q91X51 Mouse
- SwissProt: O35254 Rat
- Unigene: 613614 Human
- Unigene: 104789 Mouse
- Unigene: 144458 Rat
see all
Target information above from: UniProt accession
Q9BQQ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- FLJ23443 antibodyGolgi peripheral membrane protein p65 antibodyGolgi phosphoprotein 5 antibody
- Golgi reassembly and stacking protein 1 antibodyGolgi reassembly and stacking protein 65 kDa antibodyGolgi reassembly stacking protein 1 65kDa antibodyGolgi reassembly stacking protein 1 antibodyGolgi reassembly stacking protein of 65 kDa antibodyGolgi reassembly-stacking protein 1 antibodyGolgi reassembly-stacking protein of 65 kDa antibodyGOLPH 5 antibodyGOLPH5 antibodyGorasp 1 antibodyGorasp1 antibodyGORS1_HUMAN antibodyGRASP 65 antibodyGRASP65 antibodyMGC118894 antibodyMGC118897 antibodyP65 antibody
see all
Anti-GRASP65 antibody images
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Immunocytochemistry/ Immunofluorescence - GRASP65 antibody (ab30315)This image is courtesy of an Abreview submitted by Mr Carl HobbsGRASP65 antibody (ab30315) labelling in mouse Cor1 cell cells. Cor1 cells were fixed with formaldehyde and permeabilised. 1% BSA used as blocking agent for 10 mins. Primary antibody ab30315 incubated at rt°C at 1/5000 for 2 hours in TBS/BSA/azide/Triton. Secondary Antibody Name: anti rabbit IgG Alexa Fluor® 594 1/1000. ab30315 in Cor1 cells appears to localise discretely to an intracellular compartment that could be the Golgi Appparatus. This pattern is very similar to that in this publication: http://jcb.rupress.org/cgi/content/full/156/3/411-a -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRASP65 antibody (ab30315)Image courtesy of an Abreview submitted by Carl Hobbs.ab30315 staining GRASP65 in rat hippocampal tissue (sub-region) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.0. Samples were then blocked with 1% BSA for 10 minutes at room temperature followed by incubation with the primary antibody at a 1/6000 dilution for 16 hours. A biotin-conjugated anti-rabbit was used as secondary antibody at a 1/300 dilution. Nuclei are lightly counterstained with Haemalum. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GRASP65 antibody (ab30315)Image from Matsuki T et al., PLoS One. 2012;7(2):e31152. Epub 2012 Feb 15. Fig 5.; doi:10.1371/journal.pone.0031152; February 15, 2012, PLoS ONE 7(2): e31152.ab30315, staining GRASP65 (green) in murine hippocampus tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
Secions were incubated with primary antibody and an AlexaFluor®488-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
References for Anti-GRASP65 antibody (ab30315)
This product has been referenced in:
- Matsuki T et al. Identification of Stk25 as a genetic modifier of Tau phosphorylation in Dab1-mutant mice. PLoS One 7:e31152 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22355340) »
- Houghton FJ et al. Arl5b is a Golgi-localised small G protein involved in the regulation of retrograde transport. Exp Cell Res 318:464-77 (2012). ICC/IF ; Human . Read more (PubMed: 22245584) »
