Overview
- Product nameAnti-EPO antibodySee all EPO primary antibodies ...
- DescriptionRabbit polyclonal to EPO
- SpecificityNo crossreactivity to a cytokine test panel (ELISA).
- Tested applicationsELISA, WB, Neutralising, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
CHO cell derived recombinant human EPO.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 40% Glycerol, PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab30545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use a concentration of 0.5 - 1 µg/ml. Detection limit is ~1ng/well. |
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
| Neutralising | Neut: Use a concentration of 1 - 3 µg/ml. 1 hour at 37°C. The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1. |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
- Cellular localizationSecreted
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Database links
- Entrez Gene: 2056 Human
- Entrez Gene: 2056 Human
- Omim: 133170 Human
- SwissProt: P01588 Human
- SwissProt: P01588 Human
- Unigene: 2303 Human
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Alternative names
- EP antibodyEPO alpha antibodyEPO antibody
- Epoetin antibodyErythropoetin antibodyErythropoietin antibodyErythropoietin precursor antibodyMGC138142 antibody
see all
References for Anti-EPO antibody (ab30545)
This product has been referenced in:
- Rades D et al. Prognostic impact of erythropoietin expression and erythropoietin receptor expression on locoregional control and survival of patients irradiated for stage II/III non-small-cell lung cancer. Int J Radiat Oncol Biol Phys 80:499-505 (2011). IHC-P ; Human . Read more (PubMed: 20646855) »
- Medana IM et al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P ; Human . Read more (PubMed: 19930602) »
