Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099)

Overview

• Product nameAnti-67kDa Laminin Receptor antibody [MLuC5]See all 67kDa Laminin Receptor primary antibodies ...
• Description
  Mouse monoclonal [MLuC5] to 67kDa Laminin Receptor
• Tested applicationsInhibition Assay, IHC-Fr, WB, Flow Cyt, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Small cell lung carcinoma N592 live cells.

• Positive controlBreast carcinoma.
• General notesMolecular weight of Laminin Receptor = ~ 67 kDa.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
• Concentration information loading...
• Purification notesPurified from ascites by ammonium sulfate precipitation.
• Clonality Monoclonal
• Clone numberMLuC5
• IsotypeIgM
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Basal Lamina

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Extracellular Matrix

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Prions

  • Neuroscience
  • Cell Adhesion Proteins
  • ECM Proteins

  • Neuroscience
  • Cell Adhesion Proteins
  • Cytoskeletal Proteins
  • Intermediate Filaments

Applications

Target

• FunctionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
• Sequence similaritiesBelongs to the ribosomal protein S2P family.
• Post-translational
  modificationsAcylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
  Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
• Cellular localizationCell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.

Target information above from: UniProt accession P08865 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3921 Human
  • Entrez Gene: 100505031 Mouse
  • Entrez Gene: 16785 Mouse
  • Entrez Gene: 29236 Rat
  • Omim: 150370 Human
  • SwissProt: P08865 Human
  • SwissProt: P14206 Mouse
  • SwissProt: P38983 Rat

  • Unigene: 449909 Human
  • Unigene: 311972 Mouse
  • Unigene: 391708 Mouse
  • Unigene: 4071 Mouse
  • Unigene: 121972 Rat
  • Unigene: 161973 Rat
  • Unigene: 999 Rat

  see all

• Alternative names
  34/67 kDa laminin receptor antibody34/67 kDa laminin receptor antibody37 kDa laminin receptor precursor antibody

  37/67 kDa laminin receptor antibody37LRP antibody40S ribosomal protein SA antibody67 kDa laminin receptor antibody67LR antibodyColon carcinoma laminin binding protein antibodyColon carcinoma laminin-binding protein antibodyLAMBR antibodyLaminin Receptor 1 antibodyLaminin-binding protein precursor p40 antibodyLAMR 1 antibodyLamR antibodyLAMR1 antibodyLBP antibodyLBP/p40 antibodyLRP antibodyLRP/LR antibodyMultidrug resistance associated protein MGr1 Ag antibodyMultidrug resistance associated protein MGr1Ag antibodyMultidrug resistance-associated protein MGr1-Ag antibodyNEM/1CHD4 antibodyp40 antibodyRibosomal Protein SA antibodyRPSA antibodyRSSA_HUMAN antibodySA antibody

  see all

Anti-67kDa Laminin Receptor antibody [MLuC5] images

• 

  Immunocytochemistry/ Immunofluorescence - 67kDa Laminin Receptor antibody [MLuC5] (ab3099)
  ICC/IF image of ab3099 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Western blot - 67kDa Laminin Receptor antibody [MLuC5] (ab3099)
  Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099) at 1/1000 dilution + Mouse pancreatic cancer tissue lysate at 35 µg
  
  Secondary
  HRP-conjugated Sheep anti-Mouse polyclonal at 1/2000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 33 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 10 minutes

  This image is courtesy of an anonymous Abreview.

  See Abreview

• 

  Flow Cytometry - Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099)
  Overlay histogram showing MCF7 cells stained with ab3099 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3099, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.