Anti-Annexin-6/ANXA6 antibody (ab31026)
Key features and details
- Rabbit polyclonal to Annexin-6/ANXA6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-6/ANXA6 antibody
See all Annexin-6/ANXA6 primary antibodies -
Description
Rabbit polyclonal to Annexin-6/ANXA6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Annexin-6/ANXA6 aa 1-100. The exact sequence is proprietary.
Database link: P08133 -
Positive control
- IHC-P: Human L8 cells. Liver.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
|
IHC-P |
1/50.
1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/50. 1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Target
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Function
May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. -
Sequence similarities
Belongs to the annexin family.
Contains 8 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated in response to growth factor stimulation. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 309 Human
- Entrez Gene: 11749 Mouse
- Entrez Gene: 79125 Rat
- Omim: 114070 Human
- SwissProt: P08133 Human
- SwissProt: P14824 Mouse
- SwissProt: P48037 Rat
- Unigene: 412117 Human
see all -
Alternative names
- 67 kDa calelectrin antibody
- Annexin A6 antibody
- Annexin VI antibody
see all
Images
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Anti-Annexin-6/ANXA6 antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 5 seconds
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Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin-6/ANXA6 in human liver tissue by Immunohistochemistry.
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All lanes : Anti-Annexin-6/ANXA6 antibody (ab31026) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Anti-Annexin-6/ANXA6 antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells
Predicted band size: 76 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab31026 has been referenced in 16 publications.
- Sun X et al. ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction. Clin Transl Med 10:e208 (2020). PubMed: 33135350
- Demonbreun AR et al. Recombinant annexin A6 promotes membrane repair and protects against muscle injury. J Clin Invest 129:4657-4670 (2019). PubMed: 31545299
- Raouf R et al. Inhibition of somatosensory mechanotransduction by annexin A6. Sci Signal 11:N/A (2018). PubMed: 29921656
- Shimura M et al. A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. Oncotarget 9:24291-24303 (2018). PubMed: 29849941
- Spinelli C et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643