Anti-CREB antibody - ChIP Grade (ab31387)

Overview

• Product nameAnti-CREB antibody - ChIP GradeSee all CREB primary antibodies ...
• Description
  Rabbit polyclonal to CREB - ChIP Grade
• SpecificityImmunogen has 92% alignment to ATF-1
• Tested applicationsChIP, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.

• Positive controlHeLa cell extract. Human breast carcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH / Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
• Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
• Sequence similaritiesBelongs to the bZIP family.
  Contains 1 bZIP domain.
  Contains 1 KID (kinase-inducible) domain.
• Post-translational
  modificationsStimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
• Cellular localizationNucleus.

Target information above from: UniProt accession P16220 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1385 Human
  • Entrez Gene: 12912 Mouse
  • Entrez Gene: 81646 Rat
  • Omim: 123810 Human
  • SwissProt: P16220 Human
  • SwissProt: Q01147 Mouse
  • SwissProt: P15337 Rat
  • Unigene: 516646 Human

  • Unigene: 453295 Mouse
  • Unigene: 90061 Rat

  see all

• Alternative names
  Active transcription factor CREB antibodycAMP response element binding protein 1 antibodycAMP response element binding protein antibody

  cAMP responsive element binding protein 1 antibodycAMP-responsive element-binding protein 1 antibodyCREB 1 antibodyCREB antibodyCREB-1 antibodyCREB1 antibodyCREB1_HUMAN antibodyCyclic AMP-responsive element-binding protein 1 antibodyMGC9284 antibodyOTTHUMP00000163864 antibodyOTTHUMP00000163865 antibodyOTTHUMP00000206660 antibodyOTTHUMP00000206662 antibodyOTTHUMP00000206667 antibodyTransactivator protein antibody

  see all

Anti-CREB antibody - ChIP Grade images

• 

  Immunohistochemistry (Paraffin-embedded sections) - CREB antibody (ab31387)
  Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).
• 

  Western blot - CREB antibody (ab31387)
  Lane 1 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution
  Lane 2 : Anti-CREB antibody - ChIP Grade (ab31387) at 1/500 dilution (treated with synthesized peptide )
  
  Lane 1 : HeLa cell extract
  Lane 2 : HeLa cell extract
  
  
  Predicted band size : 37 kDa