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Anti-E. coli O + E. coli K antibody (ab31499)

Overview

  • Product nameAnti-E. coli O + E. coli K antibodySee all E. coli O + E. coli K primary antibodies ...
  • Description
    Rabbit polyclonal to E. coli O + E. coli K
  • SpecificityReactive with all O and K antigenic serotypes of E. coli.
  • Tested applicationsFlow Cyt, ELISA more details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Whole cell preparation E. coli, all serotypes

Properties

Applications

Our Abpromise guarantee covers the use of ab31499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: 1/100.
ELISA ELISA: 1/2000.

Target

  • RelevanceEscherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
  • Alternative names
      Capsular antigen antibodyEscherichia coli antibodyEscherichia coli K antibody
      Escherichia coli O antibodyK antigen antibodyO antigen antibodySomatic antigen antibody
    see all

References for Anti-E. coli O + E. coli K antibody (ab31499)

ab31499 has not yet been referenced specifically in any publications.

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Application Flow Cytometry
Sample Escherichia coli Cell (Bacterial over night culture of E.coli HB101)
Specification Bacterial over night culture of E.coli HB101
Preparation Cell harvesting/tissue preparation method: washed with PPS
Sample buffer: PBS containing 10% FCS, 1% BSA
Fixation PFA 0,4% after staining
Permeabilization No
Gating Strategy total events (FSC, SSC log)
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Submitted Feb 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"