Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

Overview

• Product nameAnti-VEGF Receptor 1 antibody [Y103]See all VEGF Receptor 1 primary antibodies ...
• Description
  Rabbit monoclonal [Y103] to VEGF Receptor 1
• Specificityab32152 recognises VEGF receptor 1 and its splice isoform sFlt1.
• Tested applicationsWB, IHC-P, ICC/IF, IP, Flow Cyt, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in N terminus of human VEGF Receptor 1

• Positive controlMouse brain tissue, A431 cells, skin cancer.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberY103
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • Angiogenic growth factors

  • Cancer
  • Growth factors
  • VEGF

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factors

  • Signal Transduction
  • Growth Factors/Hormones
  • VEGF

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Cardiovascular
  • Vasculature
  • Endothelium

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • VEGF
  • VEGF Receptors

Applications

Target

• FunctionReceptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
• Tissue specificityMostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
  Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P17948 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2321 Human
  • Entrez Gene: 14254 Mouse
  • Entrez Gene: 54251 Rat
  • Omim: 165070 Human
  • SwissProt: P17948 Human
  • SwissProt: P35969 Mouse
  • SwissProt: P53767 Rat
  • Unigene: 594454 Human

  • Unigene: 389712 Mouse
  • Unigene: 10239 Rat

  see all

• Alternative names
  EC 2.7.10.1 antibodyFlt 1 antibodyFLT antibody

  Flt-1 antibodyFlt1 antibodyFlt1 antibodyFms like tyrosine kinase 1 antibodyFms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibodyFms related tyrosine kinase 1 antibodyFms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibodyFms-like tyrosine kinase 1 antibodyFRT antibodySoluble VEGF receptor 1 14 antibodySoluble VEGFR1 variant 2 antibodySoluble VEGFR1 variant 21 antibodyTyrosine protein kinase FRT antibodyTyrosine protein kinase receptor FLT antibodyTyrosine-protein kinase FRT antibodyTyrosine-protein kinase receptor FLT antibodyVascular endothelial growth factor receptor 1 antibodyVascular endothelial growth factor receptor 1 Precursor antibodyVascular endothelial growth factor vascular permeability factor receptor antibodyVascular permeability factor receptor 1 antibodyVascular permeability factor receptor antibodyVEGFR 1 antibodyVEGFR-1 antibodyVEGFR1 antibodyVEGFR1 antibodyVGFR1_HUMAN antibody

  see all

Anti-VEGF Receptor 1 antibody [Y103] images

• 

  Flow Cytometry - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
  Overlay histogram showing A431 cells stained with ab32152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32152, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
• 

  Western blot - VEGF Receptor 1 antibody [Y103] (ab32152)
  Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue
  
  Predicted band size : 151 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103] (ab32152)
  Immunofluorescent analysis of VEGF receptor 1 expression in A431 cells using 1/250 ab32152.
• 

  Immunohistochemistry (Paraffin-embedded sections) - VEGF Receptor 1 antibody [Y103] (ab32152)
  Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 ab32152.Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 ab32152.
• 

  Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103] (ab32152)This image is courtesy of an anonymous Abreview.

  ab32152 at 1/200 dilution staining VEGF Receptor 1 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution.

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