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Overview

  • Product nameAnti-eIF4A3 antibodySee all eIF4A3 primary antibodies ...
  • Description
    Rabbit polyclonal to eIF4A3
  • Tested applicationsIHC-P, IP, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human eIF4A3.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab32484.)

  • Positive controlThis antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab32485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP IP: Use a concentration of 5 µg/ml.
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with eIF4A3 peptide (ab32484).

Target

  • FunctionATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.

    • Target information above from: UniProt accession P38919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ATP-dependent RNA helicase DDX48 antibodyATP-dependent RNA helicase eIF4A-3 antibodyDDX48 antibody
      DEAD box protein 48 antibodyeIF-4A-III antibodyeIF4A-III antibodyEIF4A3 antibodyeIF4AIII antibodyEukaryotic initiation factor 4A-III antibodyEukaryotic initiation factor 4A-like NUK-34 antibodyEUKARYOTIC TRANSLATION INITIATION FACTOR 4A ISOFORM 3 antibodyhNMP 265 antibodyIF4A3_HUMAN antibodyNMP 265 antibodyNMP265 antibodyNUCLEAR MATRIX PROTEIN 265 antibodyNUK34 antibody
    see all

Anti-eIF4A3 antibody images

  • Immunoprecipitation - Anti-eIF4A3 antibody (ab32485)
    Immunoprecipitation - Anti-eIF4A3 antibody (ab32485)

    eIF4A3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32485.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 47kDa; eIF4A3

  • Western blot - eIF4A3 antibody (ab32485)
    Western blot - eIF4A3 antibody (ab32485)
    Anti-eIF4A3 antibody (ab32485) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Western blot - eIF4A3 antibody (ab32485)
    Western blot - eIF4A3 antibody (ab32485)
    All lanes : Anti-eIF4A3 antibody (ab32485) at 1 µg/ml

    Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/ Immunofluorescence - eIF4A3 antibody (ab32485)
    Immunocytochemistry/ Immunofluorescence - eIF4A3 antibody (ab32485)
    ICC/IF image of ab32485 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32485, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4A3 antibody (ab32485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4A3 antibody (ab32485)
    IHC image of eIF4A3 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-eIF4A3 antibody (ab32485)

This product has been referenced in:
  • Chuang TW  et al. The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation. Mol Biol Cell 24:1-13 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23115303) »
  • Feng J  et al. A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing. Biol Reprod 79:546-61 (2008). WB ; Mouse . Read more (PubMed: 18480465) »

See all 2 Publications for this product

Publishing research using ab32485 ? Please let us know so that we can cite the reference in this datasheet

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Very weak signal and high background in WB

Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab32485 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If the protoco...

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