Anti-TCF7L2 antibody [0.T.149] (ab32873)

Overview

• Product nameAnti-TCF7L2 antibody [0.T.149]See all TCF7L2 primary antibodies ...
• Description
  Mouse monoclonal [0.T.149] to TCF7L2
• SpecificityRecognizes TCF4 at 66kD in Cos1 cells expressing transfected TCF4
• Tested applicationsELISA, IHC-P, ICC/IF, IP, WB, EMSA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  His-tagged fusion protein, corresponding to amino acids 31-331 of Human TCF4

• Positive controlSW620 whole cell lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number0.T.149
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Nuclear

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HMG Box

Applications

Target

• FunctionParticipates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
• Tissue specificityDetected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
• Involvement in diseaseNote=Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
  Genetic variations in TCF7L2 are associated with susceptibility to non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
• Sequence similaritiesBelongs to the TCF/LEF family.
  Contains 1 HMG box DNA-binding domain.
• Developmental stageHighly expressed in crypt regions and barely detectable in villi in epithelium from fetal small intestine at week 16. At week 22 expression in villi had increased strongly.
• DomainThe promoter-specific activation domain interacts with the transcriptional coactivator EP300.
• Post-translational
  modificationsIn vitro, phosphorylated by TNIK.
  Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylated by AXAM/SENP2. Sumoylation/desumoylation regulates TCF4 transcription activity in the Wnt signaling pathway without altering interaction with CTNNB1 nor binding DNA.
• Cellular localizationNucleus > PML body. Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Target information above from: UniProt accession Q9NQB0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6934 Human
  • Entrez Gene: 6934 Human
  • Entrez Gene: 6934 Human
  • Entrez Gene: 21416 Mouse
  • Omim: 602228 Human
  • SwissProt: Q9NQB0 Human
  • SwissProt: Q9NQB0 Human
  • SwissProt: Q9NQB0 Human

  • SwissProt: Q924A0 Mouse
  • Unigene: 593995 Human
  • Unigene: 139815 Mouse

  see all

• Alternative names
  E2 2 antibodyHMG box transcription factor 4 antibodyhTCF 4 antibody

  hTCF-4 antibodyImmunoglobulin transcription factor 2 antibodyITF2 antibodySEF2 1 antibodySEF2 1A antibodySEF2 1B antibodySEF2 antibodyT cell factor 4 antibodyT cell specific HMG box antibodyT cell specific transcription factor 4 antibodyT-cell factor 4 antibodyT-cell-specific transcription factor 4 antibodyTCF 4 antibodyTCF-4 antibodyTCF4 antibodyTCF7L2 antibodyTCF7L2 protein antibodyTF7L2_HUMAN antibodyTranscription factor 4 antibodyTranscription factor 7 like 2 antibodyTranscription factor 7 like 2 T cell specific HMG box antibodyTranscription factor 7-like 2 antibody

  see all

Anti-TCF7L2 antibody [0.T.149] images

• 

  Western blot - TCF7L2 antibody [0.T.149] (ab32873)
  All lanes : Anti-TCF7L2 antibody [0.T.149] (ab32873) at 4 µg/ml
  
  Lane 1 : Cos-1 cells
  Lane 2 : Cos-1 cells transfected with TCF7L2 cDNA in pUSE
  
  
  Predicted band size : 71 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.