Anti-AGRP antibody (ab32882)

Overview

• Product nameAnti-AGRP antibodySee all AGRP primary antibodies ...
• Description
  Goat polyclonal to AGRP
• SpecificityThis antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531).
• Tested applicationsIHC-FoFr more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Dog, Chimpanzee
• Immunogen

  Synthetic peptide: (C)RKLGTAMNPCSRT, corresponding to C terminal amino acids 120-132 of Human AGRP

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Other

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Neuroscience
  • Neurology process
  • Metabolism

  • Neuroscience
  • Endocrine system
  • Hypothalamic pituitary adrenal axis

Applications

Target

• FunctionPlays a role in weight homeostasis. Plays a role in the central control of feeding. Reduces food intake. Inhibits cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Acts primarily on MC3R and MC4R. Has very low activity with MC5R.
• Tissue specificityExpressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
• Involvement in diseaseGenetic variations in AGRP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
• Sequence similaritiesContains 1 agouti domain.
• DomainThe presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
• Cellular localizationSecreted. Golgi apparatus lumen.

Target information above from: UniProt accession O00253 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 181 Human
  • Entrez Gene: 11604 Mouse
  • Omim: 602311 Human
  • SwissProt: O00253 Human
  • SwissProt: P56473 Mouse
  • Unigene: 104633 Human
  • Unigene: 441696 Mouse

• Alternative names
  Agouti Related Protein Homolog antibodyAgouti-related protein antibodyAgrp antibody

  AGRP_HUMAN antibodyAGRT antibodyART antibodyASIP2 antibody

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Anti-AGRP antibody images

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-AGRP antibody (ab32882)Image courtesy of Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary
  Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining AGRP with ab32882 at 0.05 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).