Anti-TRP1 antibody [TA99] (ab3312)
- Product nameAnti-TRP1 antibody [TA99]See all TRP1 primary antibodies ...
- DescriptionMouse monoclonal [TA99] to TRP1
- Specificityab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
- Tested applicationsIHC-Fr, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
SK-MEL-23 Melanoma cell line.
- Positive controlIn Western Blot, this antibody gave a positive signal in human skin tissue lysate.
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberTA99
Our Abpromise guarantee covers the use of ab3312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionOxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
- Tissue specificityPigment cells.
- PathwayPigment biosynthesis; melanin biosynthesis.
- Involvement in diseaseDefects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
- Sequence similaritiesBelongs to the tyrosinase family.
- Cellular localizationMelanosome membrane.
- 5 6 dihydroxyindole 2 carboxylic acid oxidase antibody5 6 dihydroxyindole 2 carboxylic acid oxidase precursor antibody5 antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibodyAssociated with iris pigmentation antibodyb protein antibodyCAS 2 antibodyCAS2 antibodyCatalase B antibodyCatalaseB antibodyCATB antibodyDHICA oxidase antibodyGlycoprotein 75 antibodyGlycoprotein75 antibodyGP 75 antibodyGP75 antibodyMelanoma antigen gp75 antibodyTRP 1 antibodyTRP antibodyTRP-1 antibodyTRP1 antibodyTyrosinase related protein 1 antibodyTyrosinase-related protein 1 antibodyTYRP antibodyTYRP1 antibodyTYRP1_HUMAN antibodyTYRRP antibody
Anti-TRP1 antibody [TA99] images
Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml +
Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 20 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 72 kDa
Observed band size : 72 kDa
Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
ab3312 staining TRP1 in B16 mouse melanoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% goat serum for 1 hour at 22°C. The primary antibody was diluted 1/200 in 5% goat serum/1% BSA in PBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Mouse polyclonal, diluted 1/500.
References for Anti-TRP1 antibody [TA99] (ab3312)
This product has been referenced in:
- Strunnikova NV et al. Transcriptome analysis and molecular signature of human retinal pigment epithelium. Hum Mol Genet 19:2468-86 (2010). WB ; Human . Read more (PubMed: 20360305) »
- Delevoye C et al. AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis. J Cell Biol 187:247-64 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19841138) »