Anti-TRP1 antibody [TA99] (ab3312)

Overview

• Product nameAnti-TRP1 antibody [TA99]See all TRP1 primary antibodies ...
• Description
  Mouse monoclonal [TA99] to TRP1
• Specificityab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
• Tested applicationsIHC-Fr, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  SK-MEL-23 Melanoma cell line.

• Positive controlIn Western Blot, this antibody gave a positive signal in human skin tissue lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferpH: 7.40
  Preservative: 0.1% Sodium azide
  Constituent: 0.01% PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberTA99
• IsotypeIgG2a
• Research Areas
  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Tags & Cell Markers
  • Cell Type Markers
  • Other Cell Types

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• FunctionOxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
• Tissue specificityPigment cells.
• PathwayPigment biosynthesis; melanin biosynthesis.
• Involvement in diseaseDefects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
• Sequence similaritiesBelongs to the tyrosinase family.
• Cellular localizationMelanosome membrane.

Target information above from: UniProt accession P17643 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7306 Human
  • Entrez Gene: 22178 Mouse
  • Omim: 115501 Human
  • SwissProt: P17643 Human
  • SwissProt: P07147 Mouse
  • Unigene: 270279 Human
  • Unigene: 30438 Mouse

• Alternative names
  5 6 dihydroxyindole 2 carboxylic acid oxidase antibody5 6 dihydroxyindole 2 carboxylic acid oxidase precursor antibody5 antibody

  6-dihydroxyindole-2-carboxylic acid oxidase antibodyAssociated with iris pigmentation antibodyb protein antibodyCAS 2 antibodyCAS2 antibodyCatalase B antibodyCatalaseB antibodyCATB antibodyDHICA oxidase antibodyGlycoprotein 75 antibodyGlycoprotein75 antibodyGP 75 antibodyGP75 antibodyMelanoma antigen gp75 antibodyTRP 1 antibodyTRP antibodyTRP-1 antibodyTRP1 antibodyTyrosinase related protein 1 antibodyTyrosinase-related protein 1 antibodyTYRP antibodyTYRP1 antibodyTYRP1_HUMAN antibodyTYRRP antibody

  see all

Anti-TRP1 antibody [TA99] images

• 

  Western blot - TRP1 antibody [TA99] (ab3312)
  Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 20 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 72 kDa
  Observed band size : 72 kDa
  Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [TA99] (ab3312)This image is courtesy of an Abreview submitted by James Harris.
  ab3312 staining TRP1 in B16 mouse melanoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% goat serum for 1 hour at 22°C. The primary antibody was diluted 1/200 in 5% goat serum/1% BSA in PBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Mouse polyclonal, diluted 1/500.

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