Anti-CD8 alpha antibody [OX-8] (ab33786)

Overview

• Product nameAnti-CD8 alpha antibody [OX-8]See all CD8 alpha primary antibodies ...
• Description
  Mouse monoclonal [OX-8] to CD8 alpha
• Tested applicationsBlocking, Flow Cyt, IHC-Fr, IHC-P, WB, IP, ICC/IF more details
• Species reactivity
  Reacts with: Rat
  
• Immunogen

  Rat thymocyte membrane glycoproteins.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberOX-8
• MyelomaNS1
• IsotypeIgG1
• Research Areas
  • Immunology
  • Adaptive Immunity
  • T Cells
  • Cytotoxic Cells

  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

Applications

Target

• FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
• Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
• Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
• Post-translational
  modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P01732 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 24930 Rat
  • SwissProt: P07725 Rat
  • Unigene: 10306 Rat

• Alternative names
  CD 8a antibodyCD8 alpha chain antibodyCD8 antibody

  CD8 antigen alpha chain antibodyCD8 antigen alpha polypeptide antibodyCD8 antigen alpha polypeptide (p32) antibodyCD8a antibodyCD8a antigen antibodyCD8a molecule antibodyCD8A_HUMAN antibodyLeu 2 antibodyLeu2 antibodyLeu2 T lymphocyte antigen antibodyMAL antibodyOKT8 T cell antigen antibodyOTTHUMP00000160760 antibodyOTTHUMP00000160764 antibodyOTTHUMP00000203528 antibodyOTTHUMP00000203721 antibodyp32 antibodyT cell antigen Leu2 antibodyT cell co receptor antibodyT cell coreceptor antibodyT cell surface glycoprotein CD8 alpha chain antibodyT lymphocyte differentiation antigen T8/Leu 2 antibodyT lymphocyte differentiation antigen T8/Leu2 antibodyT-cell surface glycoprotein CD8 alpha chain antibodyT-lymphocyte differentiation antigen T8/Leu-2 antibodyT8 T cell antigen antibody

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Anti-CD8 alpha antibody [OX-8] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)Image courtesy of an anonymous Abreview.
  ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)Image courtesy of an anonymous Abreview.
  ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Frozen sections).
  
  Tissue was fixed in acetone, blocked with 5% serum for 30 minutes at 25°C, then incubated with ab33786 at a 1/200 for 1 hour 30 minutes at 25°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal, used at a 1/300.

  See Abreview