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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab34307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).

    This antibody does not appear to be suitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • Cellular localizationNuclear and cytoplasmic.
    • Database links
    • Alternative names
        2810406C15Rik antibody2810465G24Rik antibodyCAP-D2 antibody
        CAPD2 antibodyCEP2 antibodyCEP250 antibodyChromosome condensation-related SMC-associated protein 1 antibodyChromosome-associated protein D2 antibodyCNAP1 antibodyCND1 antibodyCondensin antibodyeg7 antibodyhCAP-D2 antibodyhCAPD2 antibodyKIAA0159 antibodymKIAA0159 antibodyNCAPD2 antibodynon-SMC condensin I complex, subunit D2 antibody
      see all

    Anti-CNAP1 antibody images

    • All lanes : Anti-CNAP1 antibody (ab34307) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg

      Secondary
      IR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    References for Anti-CNAP1 antibody (ab34307)

    ab34307 has not yet been referenced specifically in any publications.

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
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    Submitted Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"