Anti-Endostatin/COL18A1 antibody (ab3453)
Key features and details
- Rabbit polyclonal to Endostatin/COL18A1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Cat, Human, Non human primates
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Endostatin/COL18A1 antibody
See all Endostatin/COL18A1 primary antibodies -
Description
Rabbit polyclonal to Endostatin/COL18A1 -
Host species
Rabbit -
Specificity
Detects recombinant human Endostatin/COL18A1.
-
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Cat, Human, Non human primates -
Immunogen
Synthetic peptide corresponding to Human Endostatin/COL18A1 aa 129-142.
Sequence:RRLM/TESYCETWRTE
(Peptide available asab4983) -
General notes
ab3453 has been recombinant only tested in Western blot.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/200 - 1/1000.
|
|
IHC-P |
1/100 - 1/500.
|
Notes |
---|
ICC/IF
1/200 - 1/1000. |
IHC-P
1/100 - 1/500. |
Target
-
Function
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. -
Tissue specificity
Present in multiple organs with highest levels in liver, lung and kidney. -
Involvement in disease
Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. -
Sequence similarities
Belongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 1306 Human
- Entrez Gene: 80781 Human
- Entrez Gene: 12819 Mouse
- Entrez Gene: 12822 Mouse
- Omim: 120328 Human
- SwissProt: P39059 Human
- SwissProt: P39060 Human
- SwissProt: O35206 Mouse
see all -
Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
see all
Images
-
ab3453 labelling Endostatin/COL18A1 (green) in 293T cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.
-
ab3453 labelling Endostatin/COL18A1 (green) in A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.
-
ab3453 labelling Endostatin/COL18A1 (green) in NIH-3T3 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.
-
ab3453 labelling Endostatin/COL18A1 in the secretion of Human kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
-
ab3453 labelling Endostatin/COL18A1 in the secretion of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
-
ab3453 labelling Endostatin/COL18A1 in the secretion of Mouse kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab3453 has been referenced in 4 publications.
- Lee YH et al. Urinary chemokine C-X-C motif ligand 16 and endostatin as predictors of tubulointerstitial fibrosis in patients with advanced diabetic kidney disease. Nephrol Dial Transplant 36:295-305 (2021). PubMed: 31598726
- Zhu L et al. Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA-20a-mediated activation of the PTEN/PI3K/AKT signaling pathway. Int J Mol Med 46:2035-2046 (2020). PubMed: 33125088
- Battinelli EM et al. Megakaryocytes package contents into separate a-granules that are differentially distributed in platelets. Blood Adv 3:3092-3098 (2019). PubMed: 31648331
- Mohajeri A et al. Effect of Culture Condition Variables on Human Endostatin Gene Expression in Escherichia coli Using Response Surface Methodology. Jundishapur J Microbiol 9:e34091 (2016). PubMed: 27800134