Anti-Calsequestrin antibody (ab3516)
- Product nameAnti-Calsequestrin antibodySee all Calsequestrin primary antibodies ...
- DescriptionRabbit polyclonal to Calsequestrin
- SpecificityThis antibody detects Calsequestrin. It recognizes both cardiac and skeletal muscle Calsequestrin.
- Tested applicationsIP, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Dog, Human, Pig, Rainbow Trout
Purified canine cardiac Calsequestrin.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab3516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||IP: Use at an assay dependent dilution.|
|WB||WB: 1/2500. This antibody detects an ~55 kDa protein representing Calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be Calsequestrin-like proteins.|
|IHC-P||IHC-P: Use a concentration of 1 µg/ml.|
- FunctionCalsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. Also binds laminin.
- Sequence similaritiesBelongs to the calsequestrin family.
- Cellular localizationSarcoplasmic reticulum lumen. Mitochondrion. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294.
- Entrez Gene: 445005 Dog
- Entrez Gene: 845 Human
- Entrez Gene: 844 Human
- Entrez Gene: 12373 Mouse
- Entrez Gene: 12372 Mouse
- Entrez Gene: 397436 Pig
- Entrez Gene: 399506 Pig
- Entrez Gene: 29209 Rat
- Omim: 114251 Human
- Omim: 114250 Human
- SwissProt: P12637 Dog
- SwissProt: O14958 Human
- SwissProt: P31415 Human
- SwissProt: O09161 Mouse
- SwissProt: O09165 Mouse
- SwissProt: P51868 Rat
- SwissProt: P19633 Rat
- Unigene: 57975 Human
- Unigene: 632476 Human
- Unigene: 12829 Mouse
- Calmitin antibodyCalsequestrin 1 antibodyCalsequestrin 1 fast twitch skeletal muscle antibody
- Calsequestrin 2 antibodyCalsequestrin 2 fast twitch cardiac muscle antibodyCalsequestrin antibodyCalsequestrin cardiac muscle isoform antibodyCalsequestrin skeletal muscle isoform antibodyCalsequestrin-1 antibodyCardiac calsequestrin 2 antibodyCardiac calsequestrin antibodyCASQ 1 antibodyCASQ 2 antibodyCASQ1 antibodyCASQ1_HUMAN antibodyCASQ2 antibodyCSQ1 antibodyFLJ26321 antibodyPDIB1 antibodyPDIB2 antibodySkeletal muscle calsequestrin 1 antibodyskeletal muscle isoform antibody
Anti-Calsequestrin antibody images
ab3516 (1µg/ml) staining Calsequestrin in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of discrete organelles within the cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Calsequestrin antibody (ab3516)
This product has been referenced in:
- Haas S et al. DIGE proteome analysis reveals suitability of ischemic cardiac in vitro model for studying cellular response to acute ischemia and regeneration. PLoS One 7:e31669 (2012). WB ; Mouse . Read more (PubMed: 22384053) »
- Mishra S et al. Post-transcriptional silencing of SCN1B and SCN2B genes modulates late sodium current in cardiac myocytes from normal dogs and dogs with chronic heart failure. Am J Physiol Heart Circ Physiol 301:H1596-605 (2011). WB ; Dog . Read more (PubMed: 21705762) »