Anti-MSL1v1 antibody (ab35160)

Overview

• Product nameAnti-MSL1v1 antibodySee all MSL1v1 primary antibodies ...
• Description
  Rabbit polyclonal to MSL1v1

  This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MSL1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab38386.)

• Positive controlThis antibody gave a positive result in Human Ovary Tissue Lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Histones
  • H4
  • Acetylated

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• Tissue specificityExpressed in the brain.

Target information above from: UniProt accession Q7Z3B3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 284058 Human
  • Entrez Gene: 76719 Mouse
  • SwissProt: Q7Z3B3 Human
  • SwissProt: Q80TG1 Mouse
  • Unigene: 648744 Human
  • Unigene: 136418 Mouse

• Alternative names
  CENP-36 antibodycentromere protein 36 antibodyDKFZp686P06109 antibody

  DKFZp727C091 antibodyhypothetical protein LOC284058 antibodyK1267_HUMAN antibodyKIAA1267 antibodymale-specific lethal 1 homolog antibodyMGC102843 antibodyMLL1/MLL complex subunit KIAA1267 antibodyMSL1v1 antibodyNSL1 antibody

  see all

Anti-MSL1v1 antibody images

• 

  Western blot - MSL1 antibody (ab35160)
  Anti-MSL1v1 antibody (ab35160) at 1/250 dilution + Ovary (Human) Tissue Lysate at 10 µg
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 121 kDa
  Observed band size : 210 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - MSL1v1 antibody (ab35160)

  ICC/IF image of ab35160 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab35160, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.