Anti-GNAI1 + GNAI2 antibody (ab3522)
Key features and details
- Rabbit polyclonal to GNAI1 + GNAI2
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GNAI1 + GNAI2 antibody -
Description
Rabbit polyclonal to GNAI1 + GNAI2 -
Host species
Rabbit -
Specificity
Similarity with other sequences from the G protein family is predicted but has not being tested empirically.
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Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish -
Immunogen
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Positive control
- IHC-P: Human tonsil tissue. ICC: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 2 µg/ml. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. - Information by UniProt
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Database links
- Entrez Gene: 2770 Human
- Entrez Gene: 2771 Human
- Entrez Gene: 380034 Xenopus laevis
- Entrez Gene: 399463 Xenopus laevis
- Omim: 139310 Human
- Omim: 139360 Human
- SwissProt: P63097 Cow
- SwissProt: 38401 Guinea pig
see all -
Alternative names
- Adenylate cyclase-inhibiting G alpha protein antibody
- Gi antibody
- Gi1 protein alpha subunit antibody
see all
Images
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SH-SY5Y cells stained for GNAI1 (green) using ab3522 at 2 µg/mL in immunocytochemical analysis. The nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI, F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel e shows the no primary antibody control.
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IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (5)
ab3522 has been referenced in 5 publications.
- Pagano Zottola AC et al. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells 11:N/A (2022). PubMed: 36010658
- Vosahlikova M et al. Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of d-opioid receptor amount and function. Biochem Pharmacol 154:452-463 (2018). PubMed: 29883706
- Cechova K et al. Up-regulation of µ-, d- and ?-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes. J Neuroimmunol 321:12-23 (2018). PubMed: 29957382
- Doi M et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). WB . PubMed: 26882873
- Ufer C & Germack R Cross-regulation between beta1- and beta3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes. Br J Pharmacol 158:300-13 (2009). WB ; Rat . PubMed: 19719783