Overview
- Product nameAnti-PMCA1 antibodySee all PMCA1 primary antibodies ...
- DescriptionRabbit polyclonal to PMCA1
- SpecificityDetects PMCA 1a and 1b ATPase.
- Tested applicationsICC/IF, ELISA, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human - Immunogen
Synthetic Peptide: A(5)NNSVAYSGVKNSIKEAN(22). Immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA 1 ATPase protein. This sequence is completely conserved between rat and human PMCA 1 ATPase.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS, 1 mg/ml BSA, 0.05% sodium azide
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab3528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/300. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| WB | WB: 1/1000. This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
- Tissue specificityIsoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.
- Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
- DomainThe calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E.
- Cellular localizationCell membrane.
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Database links
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P11505 Rat
- Unigene: 506276 Human
- Unigene: 7208 Rat
Target information above from: UniProt accession
P20020
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AT2B1_HUMAN antibodyATP2B1 antibodyATPase Ca++ transporting plasma membrane 1 antibody
- Plasma membrane calcium ATPase 1 antibodyPlasma membrane calcium ATPase isoform 1 antibodyPlasma membrane calcium pump isoform 1 antibodyPlasma membrane calcium transporting ATPase 1 antibodyPlasma membrane calcium-transporting ATPase 1 antibodyPMCA 1 antibodyPMCA1 antibody
see all
Anti-PMCA1 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calcium Pump PMCA1 ATPase antibody (ab3528)This image is courtesy of an Abreview submitted by Dr R Empsonab3528 at 1/100 staining rat brain hippocacmpal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubation with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
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Western blot - Calcium Pump PMCA1 ATPase antibody (ab3528)
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 123 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutesThis image is courtesy of an Abreview submitted by Dr R Empson
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PMCA1 antibody(ab3528)Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PMCA1 antibody(ab3528)Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
References for Anti-PMCA1 antibody (ab3528)
This product has been referenced in:
- Kosiorek M et al. Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: Implications for catecholamine secretion by PC12 cells. Biochem Biophys Res Commun 411:235-40 (2011). WB ; Rat . Read more (PubMed: 21740891) »
- Mohamed TM et al. Plasma membrane calcium pump (PMCA4)/neuronal nitric oxide synthase complex regulates cardiac contractility through modulation of a compartmentalized cyclic nucleotide microdomain. J Biol Chem : (2011). WB ; Mouse . Read more (PubMed: 21965681) »
