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Overview

  • Product nameAnti-ANGPTL2 antibodySee all ANGPTL2 primary antibodies ...
  • Description
    Rabbit polyclonal to ANGPTL2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ANGPTL2.

    (Peptide available as ab36012.)

  • Positive controlThis antibody gave a positive signal in the following lysate: Tagged ANGPTL2 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab35574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 79 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Alternative names
        Angiopoietin like 2 antibodyAngiopoietin related protein 2 antibodyAngiopoietin-like 2 antibody
        Angiopoietin-like protein 2 antibodyAngiopoietin-related protein 2 antibodyANGL2_HUMAN antibodyAngptl2 antibodyArp2 antibodyHARP antibodyMGC8889 antibody
      see all

    Anti-ANGPTL2 antibody images

    • Anti-ANGPTL2 antibody (ab35574) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References for Anti-ANGPTL2 antibody (ab35574)

    ab35574 has not yet been referenced specifically in any publications.

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    Abreviews
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse cell lysate)
    Loading amount 20 µg
    Specification Mouse cell lysate
    Gel Running Conditions Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Verified customer

    Submitted Mar 24 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Verified customer

    Submitted Mar 24 2010

    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestine, kidney)
    Loading amount 20 µg
    Specification Intestine, kidney
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Verified customer

    Submitted Mar 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"