Overview
- Product nameAnti-Aromatase antibodySee all Aromatase primary antibodies ...
- DescriptionRabbit polyclonal to Aromatase
- Tested applicationsWB, ICC/IF, IHC-Fr, IHC-P more details
- Species reactivityReacts with: Mouse, Dog, Human
Predicted to work with: Rabbit - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.
(Peptide available as ab35661.)
- Positive controlThis antibody gave a positive signal in HL60 whole cell lysate as well as the following tissue lysates: Human placenta; Mouse ovary.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab35604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ICC/IF | |
| IHC-Fr | |
| IHC-P |
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 (see Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
- Tissue specificityBrain, placenta and gonads.
- Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries. - Sequence similaritiesBelongs to the cytochrome P450 family.
- Cellular localizationMembrane.
-
Database links
- Entrez Gene: 1588 Human
- Entrez Gene: 13075 Mouse
- Omim: 107910 Human
- SwissProt: P11511 Human
- SwissProt: P28649 Mouse
- Unigene: 260074 Human
- Unigene: 478781 Mouse
- Unigene: 5199 Mouse
Target information above from: UniProt accession
P11511
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- ARO 1 antibodyARO antibodyARO1 antibody
- ARO1 antibodyAromatase antibodyCP19A_HUMAN antibodyCPV antibodyCPV1 antibodyCYAR antibodyCYP19 antibodyCYP19A1 antibodyCYPXIX antibodyCytochrome P-450AROM antibodyCytochrome P450 19A1 antibodyCytochrome P450 family 19 subfamily A polypeptide 1 antibodyCytochrome P450, family 19, subfamily A, polypeptide 1 antibodyCytochrome P450, subfamily XIX (aromatization of androgens) antibodyEstrogen synthase antibodyEstrogen synthetase antibodyFlavoprotein linked monooxygenase antibodyMGC104309 antibodyMicrosomal monooxygenase antibodyOTTHUMP00000162543 antibodyOTTHUMP00000198350 antibodyP 450AROM antibodyP450AROM antibody
see all
Anti-Aromatase antibody images
-
Immunohistochemistry (Frozen sections) - Aromatase antibody (ab35604)This image is courtesy of an Abreview submitted by Dr David Matlackab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.
-
Immunocytochemistry/ Immunofluorescence - Aromatase antibody (ab35604)ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml. -
Western blot - Aromatase antibody (ab35604)All lanes : Anti-Aromatase antibody (ab35604) at 1 µg/ml
Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) - Blocked with 5% BSA
Lane 2 : Ovary (Mouse) Tissue Lysate - Blocked with 5% BSA
Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate - Blocked with 5% BSA
Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) - Blocked with 3% Milk
Lane 5 : Ovary (Mouse) Tissue Lysate - Blocked with 3% Milk
Lane 6 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate - Blocked with 3% Milk
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1, 2, 4, 5 & 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Lane 3 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 5000 µg/ml
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
References for Anti-Aromatase antibody (ab35604)
This product has been referenced in:
- Gatson JW et al. Aromatase is increased in astrocytes in the presence of elevated pressure. Endocrinology 152:207-13 (2011). WB . Read more (PubMed: 21047944) »
- Charitidi K & Canlon B Estrogen receptors in the central auditory system of male and female mice. Neuroscience 165:923-33 (2010). Read more (PubMed: 19925852) »
