Anti-Caveolin 1 antibody (ab36152)
Overview
- Product nameAnti-Caveolin 1 antibodySee all Caveolin 1 primary antibodies ...
- DescriptionGoat polyclonal to Caveolin 1
- SpecificityNo cross-reactivity expected to Caveolin 2 and Caveolin 3.
- Tested applicationsICC/IF, IHC-FoFr, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig - Immunogen
Synthetic peptide: DELSEKQVYDAH, corresponding to Internal sequence amino acids 33-44 of Human Caveolin 1
- Positive controlHuman spleen lysate. Lysate from transfected HEK293 cells transiently expressing Caveolin 1.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab36152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. See Abreviews. |
| IHC-FoFr | IHC-FoFr: 1/100. PubMed: 18716216 |
| WB | WB: Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa). |
Target
- FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
- Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
- Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
- Sequence similaritiesBelongs to the caveolin family.
- Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. - Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
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Database links
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P33724 Dog
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- SwissProt: Q6RVA9 Pig
- SwissProt: P41350 Rat
- Unigene: 3850 Dog
- Unigene: 74034 Human
- Unigene: 28278 Mouse
- Unigene: 470537 Mouse
- Unigene: 12842 Pig
- Unigene: 22518 Rat
see all
Target information above from: UniProt accession
Q03135
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CAV 1 antibodyCAV 1 antibodyCAV antibody
- CAV1 antibodyCAV1_HUMAN antibodycaveolae protein, 22 kD antibodycaveolin 1 alpha isoform antibodycaveolin 1 beta isoform antibodyCaveolin 1 caveolae protein 22kDa antibodyCaveolin-1 antibodyCaveolin1 antibodycell growth-inhibiting protein 32 antibodyMSTP085 antibodyOTTHUMP00000025031 antibodyVIP 21 antibodyVIP 21 antibodyVIP21 antibody
see all
Anti-Caveolin 1 antibody images
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Western blot - Caveolin 1 antibody (ab36152)Anti-Caveolin 1 antibody (ab36152) at 0.3 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).
Predicted band size : 20 kDa
Observed band size : 21,25 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-Caveolin 1 antibody (ab36152)All lanes : Anti-Caveolin 1 antibody (ab36152) at 1 µg/ml
Lane 1 : HEK293 cell lysate overexpressing Caveolin 1
Lane 2 : MOCK-transfected HEK293 cell lysate
Predicted band size : 20 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
References for Anti-Caveolin 1 antibody (ab36152)
This product has been referenced in:
- Hunt CL et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . Read more (PubMed: 21047970) »
- Schmidtko A et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci 28:8568-76 (2008). IHC-FoFr ; Mouse . Read more (PubMed: 18716216) »
