Anti-Biotin antibody [3E6] (ab36406)
Key features and details
- Mouse monoclonal [3E6] to Biotin
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-Biotin antibody [3E6]
See all Biotin primary antibodies -
Description
Mouse monoclonal [3E6] to Biotin -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule - biotinylated denatured Ovalbumin.
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General notes
Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids. -
Clonality
Monoclonal -
Clone number
3E6 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms. |
ELISA |
1/20000.
Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin. |
Notes |
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WB
Use at an assay dependent concentration. In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms. |
ELISA
1/20000. Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab36406 has been referenced in 3 publications.
- Bailey EM et al. Engineered IgG1-Fc Molecules Define Valency Control of Cell Surface Fc? Receptor Inhibition and Activation in Endosomes. Front Immunol 11:617767 (2020). PubMed: 33679705
- Liu F et al. Suppression of Membranous LRP5 Recycling, Wnt/ß-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P. Cell Rep 32:108049 (2020). PubMed: 32814052
- Rosa AMM et al. Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles. Biotechnol J 14:e1800590 (2019). PubMed: 31144775