Overview
- Product nameAnti-ABCG1 antibodySee all ABCG1 primary antibodies ...
- DescriptionRabbit polyclonal to ABCG1
- Tested applicationsIHC-P, WB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat - Immunogen
Synthetic peptide, corresponding to internal sequence amino acids 300-400 of Human ABCG1 (Peptide available as ab97573.)
- Positive controlMouse peritoneal macrophages.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Applications
Our Abpromise guarantee covers the use of ab36969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| WB | WB: 1/2500. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa). |
Target
- FunctionTransporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
- Tissue specificityExpressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
- Sequence similaritiesBelongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. - Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
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Database links
- Entrez Gene: 9619 Human
- Entrez Gene: 9619 Human
- Entrez Gene: 9619 Human
- Entrez Gene: 11307 Mouse
- Entrez Gene: 85264 Rat
- Omim: 603076 Human
- SwissProt: P45844 Human
- SwissProt: P45844 Human
- SwissProt: P45844 Human
- SwissProt: Q64343 Mouse
- Unigene: 124649 Human
- Unigene: 15691 Mouse
- Unigene: 470747 Mouse
- Unigene: 8398 Rat
see all
Target information above from: UniProt accession
P45844
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ABC transporter 8 antibodyAbc8 antibodyABCG1 antibody
- ABCG1_HUMAN antibodyATP-binding cassette sub family G member 1 antibodyATP-binding cassette sub-family G member 1 antibodyATP-binding cassette transporter 8 antibodyATP-binding cassette transporter member 1 of subfamily G antibodyATP-binding cassette, sub family G WHITE member 1 antibodyhomolog of Drosophila white antibodyMGC34313 antibodyWhite protein homolog antibodyWhite protein homolog ATP binding cassette transporter 8 antibodyWHITE1 antibodywht1 antibody
see all
Anti-ABCG1 antibody images
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Western blot - ABCG1 antibody (ab36969)All lanes : Anti-ABCG1 antibody (ab36969)
Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
developed using the ECL technique
Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCG1 antibody (ab36969)ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-ABCG1 antibody (ab36969)
This product has been referenced in:
- Wang Y et al. Supplementation with cyanidin-3-O-ß-glucoside protects against hypercholesterolemia-mediated endothelial dysfunction and attenuates atherosclerosis in apolipoprotein E-deficient mice. J Nutr 142:1033-7 (2012). Mouse . Read more (PubMed: 22535762) »
- Li D et al. Adenosine monophosphate activated protein kinase regulates ABCG1-mediated oxysterol efflux from endothelial cells and protects against hypercholesterolemia-induced endothelial dysfunction. Arterioscler Thromb Vasc Biol 30:1354-62 (2010). WB ; Human . Read more (PubMed: 20395595) »
