Anti-Claspin antibody (ab3719)

Overview

• Product nameAnti-Claspin antibodySee all Claspin primary antibodies ...
• Description
  Rabbit polyclonal to Claspin
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide (Human) - which represented a portion of human Claspin encoded within exon 8.

• Positive controlHeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • Other

Applications

Target

• FunctionRequired for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
• Sequence similaritiesBelongs to the claspin family.
• DomainThe C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
• Post-translational
  modificationsPhosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
  Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9HAW4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 63967 Human
  • Omim: 605434 Human
  • SwissProt: Q9HAW4 Human
  • Unigene: 175613 Human

• Alternative names
  Claspin antibodyclaspin homolog (Xenopus laevis) antibodyClaspin homolog antibody

  Clspn antibodyCLSPN protein antibodyCLSPN_HUMAN antibodyhClaspin antibodyHu Claspin antibody

  see all

Anti-Claspin antibody images

• 

  Western blot - Claspin antibody (ab3719)
  
  
  Predicted band size : 180 kDa
  
  
  

  Sample: Hela cell lysate (20µg)

  Antibody: Affinity purified Rabbit anti-Claspin (ab3719) used at a 1 / 1000 and 1 / 2000 dilution with (+) or without (-) blocking peptide.