Anti-SIGN Related 1 antibody [ER-TR9] (ab37220)
- Product nameAnti-SIGN Related 1 antibody [ER-TR9]See all SIGN Related 1 primary antibodies ...
- DescriptionRat monoclonal [ER-TR9] to SIGN Related 1
- Tested applicationsIHC-Fr, Flow Cyt, Blocking more details
- Species reactivityReacts with: Mouse
Mouse thymic stromal cells
- Positive controlPeritoneal macrophages
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: Tissue culture supernatant
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberER-TR9
- Research Areas
Our Abpromise guarantee covers the use of ab37220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 2µg for 106 cells.|
|Blocking||BL: Use at an assay dependent dilution. Dilutions have to be made according to the amounts of SIGN-R1 to be inactivated.|
- RelevanceSIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.
- Cellular localizationSingle-pass type II membrane protein
- CD209 antigen like protein B antibodyCD209b antigen antibodyDC SIGN related protein 1 antibody
- DC SIGNR1 antibodyOtB7 antibody
Anti-SIGN Related 1 antibody [ER-TR9] images
ab37220 staining SIGN Related 1 in Mouse spleen tissue sections by Immunohistochemistry (Frozen sections). The sections were fixed with 4% PFA for 10 minutes at room temperature prior to blocking with 10% Goat serum for 30 minutes at 15°C. The primary antibody was diluted to a concentration of 4 µg/mL in PBS with 2% Goat serum and incubated with the sample for 1 hour at 15°C. An Alexa Fluor® 488-conjugated Goat anti-Rat IgM polyclonal was used as the secondary antibody, diluted 1/500.
Overlay histogram showing SP0/2 cells stained with ab37220 (red line). The cells were fixed with 4% paraformaldehyde (10min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37220, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgM, kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SP0/2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-SIGN Related 1 antibody [ER-TR9] (ab37220)
This product has been referenced in:
- Taylor PR et al. The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages. J Immunol 172:1157-62 (2004). Blocking, Flow Cyt ; Mouse . Read more (PubMed: 14707091) »
- Kretschmer K et al. The selection of marginal zone B cells differs from that of B-1a cells. J Immunol 171:6495-501 (2003). IHC-Fr ; Mouse . Read more (PubMed: 14662849) »