Anti-AK2 antibody (ab37594)

Overview

• Product nameAnti-AK2 antibodySee all AK2 primary antibodies ...
• Description
  Rabbit polyclonal to AK2
• Tested applicationsELISA, WB, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human
  
• Immunogen

  This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 1~100 of human AK2.

• Positive controlMouse kidney tissue lysate; human hepatocarcinoma tissue

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Kinases

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
• Tissue specificityPresent in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
• Involvement in diseaseDefects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
• Sequence similaritiesBelongs to the adenylate kinase family. AK2 subfamily.
• Cellular localizationMitochondrion intermembrane space.

Target information above from: UniProt accession P54819 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 204 Human
  • Entrez Gene: 11637 Mouse
  • Entrez Gene: 24184 Rat
  • Omim: 103020 Human
  • SwissProt: P54819 Human
  • SwissProt: Q9WTP6 Mouse
  • SwissProt: P29410 Rat
  • Unigene: 470907 Human

  • Unigene: 29460 Mouse
  • Unigene: 3421 Rat

  see all

• Alternative names
  Adenylate kinase 2 antibodyadenylate kinase 2, mitochondrial antibodyAdenylate kinase isoenzyme 2 antibody

  ADK2 antibodyAK 2 antibodyak2 antibodyATP AMP transphosphorylase antibodyATP AMP transphosphorylase antibodyATP-AMP transphosphorylase 2 antibodyEC 2.7.4.3 antibodyKAD2_HUMAN antibodymitochondrial antibody

  see all

Anti-AK2 antibody images

• 

  Western blot - AK2 antibody (ab37594)
  Anti-AK2 antibody (ab37594) at 1/100 dilution + Mouse kidney tissue lysate.
  
  Predicted band size : 26 kDa
  Observed band size : 26 kDa
  Additional bands at : 55 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AK2 antibody (ab37594)
  ab37594, at 1/50 dilution, staining human cancer tissue by immunohistochemistry. The hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
• 

  Immunocytochemistry/ Immunofluorescence - AK2 antibody (ab37594)This image is courtesy of an Abreview submitted by Dr Vincent Michel
  ab37594 at 1:100 dilution detecting AK2 in inner ear (cochlea)-stria vascularis cells from mouse. AK2 (dark blue) is specifically expressed in the lumen of the capillaries and terminal blood vessels (stained green with isolectin) of the stria vascularis in the mouse cochlea (at postnatal day 7 (PN7)). Blood vessels in the spiral ligament do not contain AK2. Blood vessels in the spiral ligament do not contain AK2. Cell nuclei are stained in light blue by Hoechst staining. Actin is stained using TRITC conjugated phalloidin.

  See Abreview