Anti-AK2 antibody (ab37594)
- Product nameAnti-AK2 antibodySee all AK2 primary antibodies ...
- DescriptionRabbit polyclonal to AK2
- Tested applicationsELISA, WB, IHC-P, IHC-Fr, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Cow, Human
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 1~100 of human AK2.
- Positive controlMouse kidney tissue lysate; human hepatocarcinoma tissue
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
- Concentration information loading...
- PurityProtein G purified
- Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Clonality Polyclonal
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab37594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/100 - 1/500. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||IHC-P: 1/50 - 1/100.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
- FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.
- Tissue specificityPresent in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
- Involvement in diseaseDefects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.
- Sequence similaritiesBelongs to the adenylate kinase family. AK2 subfamily.
- Cellular localizationMitochondrion intermembrane space.
- Adenylate kinase 2 antibodyadenylate kinase 2, mitochondrial antibodyAdenylate kinase isoenzyme 2 antibody
- ADK2 antibodyAK 2 antibodyak2 antibodyATP AMP transphosphorylase antibodyATP AMP transphosphorylase antibodyATP-AMP transphosphorylase 2 antibodyEC 220.127.116.11 antibodyKAD2_HUMAN antibodymitochondrial antibody
Anti-AK2 antibody images
Anti-AK2 antibody (ab37594) at 1/100 dilution + Mouse kidney tissue lysate.
Predicted band size : 26 kDa
Observed band size : 26 kDa
Additional bands at : 55 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
ab37594, at 1/50 dilution, staining human cancer tissue by immunohistochemistry. The hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
ab37594 at 1:100 dilution detecting AK2 in inner ear (cochlea)-stria vascularis cells from mouse. AK2 (dark blue) is specifically expressed in the lumen of the capillaries and terminal blood vessels (stained green with isolectin) of the stria vascularis in the mouse cochlea (at postnatal day 7 (PN7)). Blood vessels in the spiral ligament do not contain AK2. Blood vessels in the spiral ligament do not contain AK2. Cell nuclei are stained in light blue by Hoechst staining. Actin is stained using TRITC conjugated phalloidin.
References for Anti-AK2 antibody (ab37594)
This product has been referenced in:
- Lagresle-Peyrou C et al. Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nat Genet 41:106-11 (2009). IHC-Fr ; Mouse . Read more (PubMed: 19043416) »
- Zhao J et al. The novel conserved mitochondrial inner-membrane protein MTGM regulates mitochondrial morphology and cell proliferation. J Cell Sci 122:2252-62 (2009). WB ; Human . Read more (PubMed: 19535734) »