Anti-PRMT2 antibody - ChIP Grade (ab3763)

Overview

• Product nameAnti-PRMT2 antibody - ChIP GradeSee all PRMT2 primary antibodies ...
• Description
  Rabbit polyclonal to PRMT2 - ChIP Grade
• Tested applicationsWB, ELISA, IHC-P, ChIP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: AIADYAATDETQLSFLR, corresponding to amino acids 37-53 of Human PRMT 2.

  Run BLAST with Run BLAST with
• General notes

  
  

  Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.1% sodium azide
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Primary antibody notes Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation
  • Arginine Methylation

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

Applications

Target

• FunctionArginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
• Tissue specificityWidely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
• Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
  Contains 1 SH3 domain.
• Cellular localizationCytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.

Target information above from: UniProt accession P55345 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3275 Human
  • Omim: 601961 Human
  • SwissProt: P55345 Human
  • Unigene: 154163 Human

• Alternative names
  ANM2_HUMAN antibodyEC 2.1.1. antibodyhistone arginine N methyltransferase PRMT2 antibody

  Histone-arginine N-methyltransferase PRMT2 antibodyHMT 1 antibodyHMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibodyHMT1 antibodyHMT1 hnRNP methyltransferase like 1 antibodyhnRNP methyltransferase (S. cerevisiae) like 1 antibodyhnRNP methyltransferase (S. cerevisiae)-like 1 antibodyhnRNP methyltransferase like 1 antibodyHrmt1l1 antibodyMGC111373 antibodyPRMT 2 antibodyPRMT2 alpha antibodyprmt2 antibodyPRMT2 beta antibodyPRMT2 gamma antibodyPRMT2 protein antibodyPRMT2L2 antibodyProtein arginine methyltransferase 2 antibodyProtein arginine N methyltransferase 2 antibodyProtein arginine N-methyltransferase 2 antibodyZf2 antibody

  see all

Anti-PRMT2 antibody - ChIP Grade images

• 

  Western blot - PRMT2 antibody - ChIP Grade (ab3763)
  
  
  Predicted band size : 49 kDa
  
  
  Western blot analysis using whole HL60 cell lysate: PRMT2 (Arrow) was detected using ab3763. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• 

  ChIP - PRMT2 antibody - ChIP Grade (ab3763)Sylvain Daujat
   

  Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3763 to PRMT2 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 µl of ab3763 and 2x106 cells were used in each ChIP experiment.

   

  Comments:  ab3763 appears to work in ChIP. It is difficult to be sure about the relevance of the results since we do not know what we could expect. If you compare ab3667 and 3763 to PRMT2, the results are actually not too bad even if they give slightly different profiles at 30 and 60 min.

   

   
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT2 antibody - ChIP Grade (ab3763)
  ab3763 staining PRMT2 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).