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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab38204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).Can be blocked with PLAGL1 / ZAC peptide (ab39114).

Target

  • Alternative names
      DKFZp781P1017 antibodyLost on transformation 1 antibodyLost on Transformation antibody
      LOT-1 antibodyLOT1 antibodyMGC126275 antibodyMGC126276 antibodyPLAG like 1 antibodyPLAGL1 antibodyPLAL1_HUMAN antibodyPleiomorphic adenoma gene like 1 antibodyPleiomorphic adenoma gene like protein 1 antibodyPleiomorphic adenoma-like protein 1 antibodyTumor supressor ZAC antibodyZAC antibodyZAC tumor supressor antibodyZAC1 antibodyZinc finger protein PLAGL1 antibody
    see all

Anti-PLAGL1 / ZAC antibody images

  • Anti-PLAGL1 / ZAC antibody (ab38204) at 1 µg/ml + PLAGL1 recombinant protein at 0.5 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-PLAGL1 / ZAC antibody (ab38204)

ab38204 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Human Cell lysate - whole cell (HEK293 HeLa U2OS MCF7)
Loading amount 20 µg
Specification HEK293 HeLa U2OS MCF7
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Submitted Mar 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"