Recommended dilutions WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 57 kDa. When used against the reduced protein, ab39005 identifies bands at 58 Kd. Note: the conditioned media may need to be concentrated for Western blot. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.