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| | Western blot - MMP22 antibody (ab39082)
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| Product Name | MMP22 antibody - Propeptide domain |
| | See all MMP22 antibodies (4)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to MMP22 - Propeptide domain |
| Immunogen | Synthetic peptide based on the propeptide domain of the chicken MMP22 sequence.
(Peptide available as ab41118.) |
| Reacts with
(species key) | Chk |
| Specificity | This antibody is specific for MMP22, and does not react with other family members (MMP1, MMP2, MMP3, MMP9, etc.). We have a range of domain specific antibodies for this target. For a full list please see all MMP22 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non human samples. Detects bands of approximately 70kDa and 66kDa when used against the reduced protein. It is unclear if this represents alternative splicing, post translational modifications, aggregation, or some other phenomenon. (Predicted molecular weight: 53 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | Cell media from human fetal fibroblasts and mouse lung tissue. |
| Cellular localization | Secreted |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP |
| Relevance | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene lacks the signal sequence found in other MMPs, suggesting that it may function intracellularly. The substrate for the enzyme has not been identified, but it is thought that the protein may play a role in reproductive processes. This protein is encoded by the MMP22 or MMP23B gene which is part of a duplicated region of chromosome 1p36.3, which includes a second gene, MMP23A, which is almost identical.
MMP22 was first cloned from chicken fibroblasts, and named CMMP. Sequence comparisons confirmed CMMP was a metalloproteinase, but homology between chicken and human MMPs was too low to assign CMMP as a human ortholog. Several years later CMMP was given the moniker MMP22. A BLAST of chicken MMP22 finds human MMP27 as the closest human sequence. Chicken MMP22 compares most closely to human MMP27, with 59% identity (compared to 51% identity between CMMP and human MMP3). It is hazardous to guess the identity of species orthologs between chicken and human proteins: chicken MMP2 is 84% identical to human and chicken MMP13 is 75% identical to human, but chicken MMP9 is only 59% identical to human MMP9. The chicken MMP22 sequence encodes a 472 amino acid protein, with predicted molecular weight of 53.254 kD. Although MMP22 was thought to be most like human MMP3 (Stromelysin 1), chicken MMP22 protein has a quite basic predicted isoelectric point of 9.104, while human MMP3 has a predicted isoelectric point of 5.699. Human MMP27 has a predicted isoelectric point of 8.826, making it similar to CMMP. The signal sequence, and lack of a transmembrane domain lead to the assumption that MMP22 is a secreted MMP, and it is tempting to speculate that the basic isoelectric point might direct the MMP22 to interact with ECM. It is still uncertain if MMP22 is a unique MMP, or the chicken ortholog of human MMP27. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP22 antibody - Propeptide domain is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for MMP22 antibody - Propeptide domain images, references, products related to ab39082 and other tools. |
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MMP22 antibody - Propeptide domain images: |
 Western blot - MMP22 antibody (ab39082)
All lanes : MMP22 antibody - Propeptide domain (ab39082) at 1/1000 dilution
Lane 1 : Cell media from human fetal fibroblasts.
Lane 2 : Mouse lung tissue.
Predicted band size : 53 kDa
Observed band size : 66 kDa ( |
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