ADAM8 antibody - Propeptide domain (ab39152) datasheet

Product Name 

ADAM8 antibody - Propeptide domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM8 - Propeptide domain

Immunogen 

Synthetic peptide of Human ADAM8 based on the aminoterminal region (the propeptide domain, preceding the cysteine switch).

(Peptide available as ab41160.)

Reacts with 
(species key)

Hu, Rat, Pig

Specificity 

This antibody does not react with other ADAMs.

We have a range of domain specific antibodies for this target. For a full list please see all ADAM8 antibodies

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
WB: 1/1000 - 1/5000. Detects a band of approximately 70 52 kDa (predicted molecular weight: 89 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell media from human chondrosarcoma.

Cellular localization 

Cell Membrane, but the majority is shed in the extracellular space.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing

Relevance 

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3.
The function of ADAM8 is still poorly understood. ADAM8 has been shown to stimulate osteoclasts, and to be produced by bone marrow cultures stimulated by vitamin-D. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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