| · Glycerol MSDS · Sodium Azide MSDS | |
|
| Product Name | ADAM13 antibody - Propeptide domain |
| | See all ADAM13 antibodies (4)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to ADAM13 - Propeptide domain |
| Immunogen | Synthetic peptide based on the amino end of Xenopus ADAM13.(Peptide available as ab41208.) |
| Reacts with
(species key) | Xl |
| Specificity | ab39157 recognises metalloproteinase ADAM-13. We have a range of domain specific antibodies for this target. For a full list please see all ADAM13 antibodies |
| Tested applications
(see key) | WB |
|
| Abreviews | |
|
| Application notes
(see key) | Recommended dilutions
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes bands of 120 kDa (minor band), 92 kDa (major band), and cleaved products at 68 kDa, 40 kDa in reduced Western blots of cell lysates. (Predicted molecular weight: 100 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha |
| Relevance | ADAM13 was first described as a protein expressed in somatic mesoderm and neural crest cells, in developing Xenopus embryos. ADAM13 was also found in liver, heart, and intestines from adult Xenopus. ADAM13 may regulate cellular signaling via Src and Src tyrosine kinase. ADAM13 may also act as a cell attachment molecule, by binding integrins through the cysteine rich domain amoung many other roles. A member of the metalloproteinase family containing disintegrin like domains (ADAMs) the functions of ADAM13 are still poorly understood. ADAM13 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, as well as disintegrin, cysteine rich, EFG like, transmembrane and Cytoplasmic domains. ADAM13 has been shown to be proteolytically active, cleaving fibronectin after binding it to the EGF like domain. ADAM13 is also shed from cells in culture, cleaved aminoterminal from the transmembrane domain, and is released into the culture media. Shed ADAM13 is a 52 kD protein, and can form complexes with a2 macroglobulin, suggesting it is a competent protease. Xenopus ADAM13 has greatest homology with human ADAM 33 (51% identical), and is 46% identical with human or mouse ADAM12 or ADAM19. It is still unclear if any of these ADAMs are species orthologs of Xenopus ADAM13, but there are significant differences between the related sequences, suggesting that ADAM13 may be a unique protein.
The full length Xenopus ADAM13 sequence codes for a 914 amino acid protein. Predicted mass is 99.749 kD, but glycosylation and cyteine rich regions give Xenopus ADAM13 an apparent MW of 120 kD unprocessed, and 97 kD processed forms, on reduced SDS PAGE gels. ADAM13 contains a putative furin cleavage site, suggesting that a prohormone convertase cleaves the propeptide domain away from the catalytic domain. |
| | |
| Database links | The links below go to external sites and will open in a new browser window Entrez Gene | | 386623 (Xenopus laevis)
|
| Unigene | | 166 (Xenopus laevis)
|
|
|
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
| Purification notes | The antibody has been peptide affinity purified. |
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ADAM13 antibody - Propeptide domain is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
| Search PubMed (MEDLINE) for references to ADAM13 |
| |
ADAM13 antibody - Propeptide domain - more information |
Customer reviews (feedback) regarding ADAM13 antibody - Propeptide domain |
Customer enquiries (FAQ's) regarding ADAM13 antibody - Propeptide domain |
Protocols for ADAM13 antibody - Propeptide domain |
Price and availability of products related to ADAM13 antibody - Propeptide domain |
| |
ADAM13 antibody - Propeptide domain - related products: |
| |
my account
basket
help
contact us
Datasheet
References (0)
