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| | Western blot - Calpain 2 antibody - Domain III (ab39165)
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| Product Name | Calpain 2 antibody - Aminoterminal end domain III |
| | See all Calpain 2 antibodies (7)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 2 - Aminoterminal end domain III |
| Immunogen | Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2.(Peptide available as ab41302.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | ab39165 binds to reduced and non-reduced calpain 2, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). It reacts with latent and active (amino-truncated) Calpain 2. We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies |
| Tested applications
(see key) | ICC/IF, WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.
ICC/IF: Use at an assay dependent dilution from (PubMed: 17192410).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | Cell lysate from human chondrosarcoma treated with IL1 alpha. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.
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| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.
Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consi |
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