| · Glycerol MSDS · Sodium Azide MSDS | |
|
| Product Name | Calpain 2 antibody - Carboxyterminal end domain III |
| | See all Calpain 2 antibodies (7)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 2 - Carboxyterminal end domain III |
| Immunogen | Synthetic peptide based on the carboxyterminal end of Domain III, the large subunit of Human Calpain 2.(Peptide available as ab41304.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody reacts with Domain III of Calpain 2, and detects both latent and aminoprocessed (activated) Calpain 2. The antibody does not cross react with the other Calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies |
| Tested applications
(see key) | WB |
|
| Abreviews | |
|
| Application notes
(see key) | Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | Cell lysates from human chondrosarcoma treated with IL1 alpha. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.
|
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.
Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. |
| | |
| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 2 antibody - Carboxyterminal end domain III is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
| Search PubMed (MEDLINE) for references to Calpain 2 |
| |
Calpain 2 antibody - Carboxyterminal end domain III - more information |
Customer reviews (feedback) regarding Calpain 2 antibody - Carboxyterminal end domain III |
Customer enquiries (FAQ's) regarding Calpain 2 antibody - Carboxyterminal end domain III |
Protocols for Calpain 2 antibody - Carboxyterminal end domain III |
Price and availability of products related to Calpain 2 antibody - Carboxyterminal end domain III |
| |
Calpain 2 antibody - Carboxyterminal end domain III - related products: |
| |
my account
basket
help
contact us
Datasheet
References (0)
