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| Product Name | Calpain 2 antibody - Domain III |
| | See all Calpain 2 antibodies (7)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 2 - Domain III |
| Immunogen | Synthetic peptide based on the calcium binding region of Domain III, in the large subunit of Human Calpain 2.
(Peptide available as ab44836.) |
| Reacts with | Hu |
| Predicted to react
(species key) | Ms
(Due to sequence homology) |
| Specificity | This antibody binds to Calpain 2, both reduced and non reduced forms, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 - 1/5000. Predicted molecular weight: 80 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.
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| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.
Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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| Search PubMed (MEDLINE) for references to Calpain 2 |
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