| · Glycerol MSDS · Sodium Azide MSDS | |
|
| Product Name | Calpain 1 antibody - Carboxyterminal end domain IV |
| | See all Calpain 1 antibodies (10)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 1 - Carboxyterminal end domain IV |
| Immunogen | Synthetic peptide based on the carboxyterminal end of the large subunit, using the human Calpain 1 sequence.(Peptide available as ab41301.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody is specific for Calpain 1, but does not cross react with the other calpain family members (M-calpain, calpain-94, ncl-2, ncl-3, etc.). It reacts to Domain-IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. We have a range of domain specific antibodies for this target. For a full list please see all Calpain 1 antibodies |
| Tested applications
(see key) | ELISA, IP, WB |
|
| Abreviews | |
|
| Application notes
(see key) | Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects bands approximately 80kDa and 58kDa and a series of further cleaved active forms (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain like activity, thus the -pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV.
The calpains, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach specific, and muscle specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. |
| | |
| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
| Purification notes | This antibody has been peptide-affinity purified. |
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 1 antibody - Carboxyterminal end domain IV is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
| Search PubMed (MEDLINE) for references to Calpain 1 |
| |
Calpain 1 antibody - Carboxyterminal end domain IV - more information |
Customer reviews (feedback) regarding Calpain 1 antibody - Carboxyterminal end domain IV |
Customer enquiries (FAQ's) regarding Calpain 1 antibody - Carboxyterminal end domain IV |
Protocols for Calpain 1 antibody - Carboxyterminal end domain IV |
Price and availability of products related to Calpain 1 antibody - Carboxyterminal end domain IV |
| |
Calpain 1 antibody - Carboxyterminal end domain IV - related products: |
| |
| |
Calpain 1 antibody - Carboxyterminal end domain IV - other tools: |
Contact Abcam with a Technical Enquiry about Calpain 1 antibody - Carboxyterminal end domain IV |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
my account
basket
help
contact us
Datasheet
References (0)
