You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameMMP2 protein (Rat)See all MMP2 proteins and peptides ...
  • Protein descriptionMMP2 protein purified from rat lung epithelial cell culture media. The enzyme is supplied as a mixture of the zymogen and processed enzyme.
  • Expression hostNative

    • Properties

  • Purification notesThis protein was purified from rat lung epithelials, and is free of its endogenous inhibitor, TIMP2, and other matrix metalloproteinases.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, 75mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab39304 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Zymography Zymography: Use at an assay dependent concentration. The Rat MMP-2 is active and is ready for enzymatic activity if diluted into a buffer of 20 mM Tris-HCl, pH 7.4, containing 150 mM NaCl, 10 mM CaCl2, and 0.1 mM ZnCl2. It will be most active at 37 degrees C but is still active at lower temperatures.
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 74 kDa.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab38917.

    • Protein info

    • Alternative names
        72 kDa gelatinase72kD type IV collagenaseCLG 4
        CLG 4ACLG 4ACLG4CLG4ACLG4ACollagenase Type 4 alphaCollagenase Type 4 alphaCollagenase type IV AGelatinase AGelatinase alphaGelatinase alphaGelatinase neutrophilMatrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenaseMatrix Metalloproteinase 2Matrix Metalloproteinase 2Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)Matrix metalloproteinase IIMatrix metalloproteinase-2MMP 2MMP 2MMP IIMMP-2MMP2MMP2_HUMANMONANeutrophil gelatinasePEXTBE 1TBE-1
      see all
  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

    • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for MMP2 protein (Rat) (ab39304)

    This product has been referenced in:
    • Hawkins KE  et al. Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke. Mol Brain 6:14 (2013). Read more (PubMed: 23522154) »
    • Rosewell K  et al. Identification of hepsin and protein disulfide isomerase A3 as targets of gelatinolytic action in rat ovarian granulosa cells during the periovulatory period. Biol Reprod 85:858-66 (2011). Read more (PubMed: 21734266) »

    See all 2 Publications for this product

    Publishing research using ab39304 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab39304

    Displaying 1 - 9 of 9 results for Q&A

    10
    Votes: Highest First

    Yes I'll try the human MMP2 protein cat # ab39302.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

    Read More

    I tried this MMp2 protein for a western blot positive control but did not work (no MMP2 band). I loaded 3 wells with 0.05 ug, 0.1 ug, and 1.0 ug MMP2 protein respectively. I also loaded a 50 ug of rat ovarian lysate total protein in another well. The cond...

    Read More

    Thank you for taking time to contact us. I am sorry to hear this protein is not providing satisfactory results.
    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I ...

    Read More

    Will MMP2 protein (rat) ab39304 work as a positive control in western blot using the anti-MMP2 antibody cat# ab37150?

    Thank you for contacting Abcam.
    You should not have any issues using ab39304 with ab37150 as a positive control in western blot. Both are covered under our Abpromise for six months and are guaranteed to work in western blot.
    If there is anything ...

    Read More

    I am very interested in purchasing your product with catalog number ab39304 (rat MMP2 protein).

    I would like to use it as a positive control for collagen IV degradation in my samples. I wonder if you know what amount of protein (in units or mic...

    Read More

    Thanks for your interst in ab39304.

    At this time we know that this rat MMP2 protein is active and has been used in zymography. On the datasheet this is described as:

    "Zymography: Use at an assay dependent concentration. The Rat MM...

    Read More

    Did not see a band in WB with this protein. Antibody works with a mouse recombinant protein though.

    Thank you for your call today and for letting us know about the trouble with this protein.

    I had requested that a replacement vial be sent to you, but your colleague kindly called to let us know that we have previously credited your account for ...

    Read More

    Thanks for your email. I will accept thecredit note as compensation.

    Thanks for looking into this for me.

    Thank you for your reply and understanding.

    I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

    Credit note ID: #####

    ...

    Read More

    Hi,

    I purchased a MMP-2 protein (ab39304).
    After using it for zymography I found I have 2 bands when I only expected one. I believe the upper band maybe MMP-9 and the lower MMP-2. Has anyone else found this for this product? Also, would I ...

    Read More

    Thank you for your enquiry and for your patience. I am sorry to hear you have some concerns regarding this product.

    I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this infor...

    Read More

    I sent this email a week ago and I was told that this was being looked into. I was wondering if you have determined anything yet. Thanks

    Hi,

    I purchased a MMP-2 protein (ab39304).

    After using it for zymography I found I ha...

    Read More

    Thank you for your message and for your patience.

    I sincerely apologise for the delay. I have contacted our source to request the information regarding the purification of the product, but they have regrettably not yet got back to me. I will co...

    Read More

    I am looking for recombinant proteins for MMP-2 and MMP-9 for gelatinzymography.

    Do you have any and if yes how much are they?

    Thank you for contacting us.

    We do have several MMP proteins in our catalog.

    We have for MMP2:

    ab39302 (human):Full length Human MMP2 protein (purified from human fibroblast cell culture media). It has not yet been tested in...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"