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MMP9 protein (ab39308) datasheet

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· Glycerol MSDS

Product Name

MMP9 protein

Product type

Proteins and Peptides

Peptide

MMP9 from cell culture media of stimulated human fibroblasts.

Specificity

We have a range of domain specific antibodies for this target. For a full list please see all MMP9 antibodies

Tested applications
(see key)

SDS-PAGE, WB

Application notes
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 92 kDa. Dilution optimised using Chromogenic detection. This enzyme is supplied predominantly as a zymogen, with a small amount of processed (cleaved) enzyme present. The zymogen can be activated by incubation at 37 degree Celsius for 2 to 6 hours with the organomercurial compound APMA at 1 mM. The enzyme degrades denatured collagen (gelatin), and a range of extracellular matrix components in vivo. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). TIMPs have been shown to block tumor cell invasion suggesting that they act as metastasis suppressor genes.

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas. MMP9 is constitutively produced by some tumor cell lines (e.g.: HT1080, HL60, U937) but not by most quiescent cells and tissues. Treatment of cells with the phorbol ester TPA stimulates production of MMP9 in some cell types, but the low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western blotting.

Alternative names for MMP9 protein (ab39308)

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, pH 7.4

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes

Purified from cell culture media of stimulated human fibroblasts, this protein is free of its endogenous inhibitor, TIMP1, and other matrix metalloproteinases.

Form

Liquid

Concentration

Batch dependent within range: 0.06 - 0.10 mg/ml mg/ml

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP9 protein is on this datasheet. But please do contact us if you would like any reassurance!