| · Glycerol MSDS | |
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| Product Name | MMP11 protein |
| Product type | Proteins and Peptides |
| Peptide | Full length Human MMP11 protein (purified from stimulated human fibroblasts) and supplied as a mixture of zymogen and processed (cleaved)forms.
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| Specificity | We have a range of domain specific antibodies for this target. For a full list please see all MMP11 antibodies |
| Tested applications
(see key) | ELISA, SDS-PAGE, WB |
| Application notes
(see key) | Recommended dilutions
ELISA: Use at an assay dependent dilution. SDS-PAGE: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 60 kDa (Zymogen) and 50 kDa (processed form). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP |
| Relevance | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1 proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.
MMP11, also known as stromelysin 3, is overexpressed in the stromal cells of invasive breast carcinomas but not in the stromal cells surrounding benign breast fibroadenomas. MMP11 is secreted in its proenzyme from and the zymogen has a mass of 64 kDa. The enzyme is rapidly activated to a 58 kDa form, a 45-47 kDa size, then to a 28 kDa final active form. MMP11 is not constitutively produced by most cell types. It has been found in lung fibroblasts and gingival fibroblasts when stimulated with EGF, as well as breast tumor and osteocarcinoma cell lines. |
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| Purity | >95% by SDS-PAGE |
| Storage buffer | Preservative: None
Constituents: 50% Glycerol, 0.02% Brij 35, 5mM Calcium chloride, 100mM Sodium chloride, 10mM Tris HCl, pH 7.4 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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| Purification notes | This protein is free of contaminating MMPs or TIMPs. |
| Form | Liquid |
| Concentration | 0.030 mg/ml |
| Storage instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP11 protein is on this datasheet. But please do contact us if you would like any reassurance! |
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