| · Glycerol MSDS · Sodium Azide MSDS | |
|
| Product Name | MMP13 protein |
| Product type | Proteins and Peptides |
| Peptide | Full length Human MMP13 protein (purified from stimulated human fibroblasts). The enzyme is supplied as a mixture of the zymogen and processed protein. |
| Specificity | We have a range of domain specific antibodies for this target. For a full list please see all MMP13 antibodies |
| Tested applications
(see key) | EIA-Ctrl, SDS-PAGE, WB |
| Application notes
(see key) | Recommended dilutions
EIA-Ctrl: Use at an assay dependent dilution. SDS-PAGE: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight (unprocessed precursor): 54 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Research areas | Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP |
| Relevance | Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack. |
| | |
| Storage buffer | Preservative: 0.01% Sodium Azide
Constituents: 10mM MES, 50% Glycerol, 0.025% Brij 35, 5mM Calcium chloride, 100mM Sodium chloride, pH 5.5 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
| Purification notes | The enzyme is purified from stimulated human fibroblasts, and is free of contaminating MMPs or TIMPs. |
| Form | Liquid |
| Concentration | 0.100 mg/ml |
| Storage instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP13 protein is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
| Search PubMed (MEDLINE) for references to MMP13 |
| |
MMP13 protein - more information |
Customer reviews (feedback) regarding MMP13 protein |
Customer enquiries (FAQ's) regarding MMP13 protein |
Protocols for MMP13 protein |
Price and availability of products related to MMP13 protein |
| |
MMP13 protein - other tools: |
Contact Abcam with a Technical Enquiry about MMP13 protein |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
my account
basket
help
contact us
Datasheet
References (0)