| · Glycerol MSDS | |
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| Product Name | TIMP1 protein |
| Product type | Proteins and Peptides |
| Peptide | Full length Human TIMP1 protein (purified from stimulated human fibroblasts).
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| Specificity | We have a range of domain specific antibodies for this target. For a full list please see all TIMP1 antibodies |
| Tested applications
(see key) | WB |
| Application notes
(see key) | Recommended dilutions
WB standard: Use at an assay dependent dilution. Produces a band of approximately 29 kDa due to glycosylation. (Predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP |
| Relevance | Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).
TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells.
TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. |
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| Purity | >95% by SDS-PAGE |
| Storage buffer | Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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| Purification notes | TIMP1 is purified from stimulated human fibroblasts, and is essentially free of contaminating MMPs or other known TIMPs. |
| Form | Liquid |
| Concentration | 0.100 mg/ml |
| Storage instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIMP1 protein is on this datasheet. But please do contact us if you would like any reassurance! |
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| Search PubMed (MEDLINE) for references to TIMP1 |
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