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| | Western blot - PCSK7 antibody - Aminoterminal end (ab39874)
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| Product Name | PCSK7 antibody - Aminoterminal end |
| | See all PCSK7 antibodies (4)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to PCSK7 - Aminoterminal end |
| Immunogen | Synthetic peptide corresponding to the amino end of activated Human PCSK7.
(Peptide available as ab41415.) |
| Reacts with
(species key) | Hu |
| Specificity | ab39874 recognises the Amino end of mature enzyme PCSK7. We have a range of domain specific antibodies for this target. For a full list please see all PCSK7 antibodies |
| Tested applications
(see key) | WB |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. The message for PC7-A encodes a 591 amino acid protein with a predicted mass of 64.4 kDa, and a pI of 5.9. The PC7-B protein has an apparent mass of 101 kDa on reduced SDS PAGE gels, and is processed to a secreted 89 kDa form. Predicted molecular weight: 86 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | The longer form is thought to be bound in the Golgi, and the shorter form secreted through dense core secretory granules. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases |
| Relevance | PC7 was the first described in the rat pituitary, and found to be expressed in many tissues including brain, adrenal gland, gut, ovary, testis, kidney, liver lung, thyroid and pancreas. A literature alias is C8 or PC8 . Two splice variants are reported; a 591 amino acid sequence (PC7A) and a 785 amino acid membrane-associated form (PC7B). The longer form is thought to be bound in the Golgi, and the shorter form secreted through dense core secretory granules. PC7 has been shown to cleave parathyroid hormone and Tumor Necrosis Factor aloha converting enzyme (TACE, ADAM17). Like furin, PC1, PC2 and PACE4, PC7 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs (subtilisin-like proprotein convertases), with PC7 getting the SPC7 moniker.
PC7 structure contains a propeptide domain, a catalytic domain and an RGD containing cysteine-rich domain (homo-B). The longer sequence contains an extended cysteine-rich domain. The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC7 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage. PC7A resides mainly in the dense core secretory vesicles and the TGN, and is cycled to the surface, with some of the PC7 being secreted from the cells. PC7 has been reported to be elevated in tumor cell lines and to increase tumor progression. The message for PC7A encodes a 591 amino acid protein with a predicted mass of 64.4 kDa, and a pI of 5.9. The PC7B protein has an apparent mass of 101 kDa on reduced SDS PAGE gels, and is processed to a secreted 89 kDa form. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PCSK7 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for PCSK7 antibody - Aminoterminal end images, references, products related to ab39874 and other tools. |
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PCSK7 antibody - Aminoterminal end images: |
 Western blot - PCSK7 antibody - Aminoterminal end (ab39874)
All lanes : PCSK7 antibody - Aminoterminal end (ab39874) at 1/1000 dilution
Lane 1 : Recombinant human PC7 at 0.05 µg
Lane 2 : Recombinant human PC7 at 0.01 µg
Lane 3 : Recombinant human PC7 at 0.001 µg
Predicted band size : 86 kDa
Observed band size : 89 kDa (why is the actual band size different from the predicted?)
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| Search PubMed (MEDLINE) for references to PCSK7 |
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