Anti-Calreticulin antibody - ER Marker (ab4)

Overview

• Product nameAnti-Calreticulin antibody - ER MarkerSee all Calreticulin primary antibodies ...
• Description
  Rabbit polyclonal to Calreticulin - ER Marker
• SpecificityThis antibody is specific for calreticulin.
• Tested applicationsICC/IF, IHC-P, WB, IP, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Hamster, Cow, Human, Zebrafish, African Green Monkey
  
• Immunogen

  Fusion protein, containing amino acids 154-347 of Mouse Calreticulin.

• Positive controlIC: COS7 and HCT29 cells WB: H4IIE cell lysate, NBL-1 bovine renal epithelial cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • ER

Applications

Target

• FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
• Sequence similaritiesBelongs to the calreticulin family.
• DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
  The interaction with glycans occurs through a binding site in the globular lectin domain.
  The zinc binding sites are localized to the N-domain.
  Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
• Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.

Target information above from: UniProt accession P27797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 281036 Cow
  • Entrez Gene: 811 Human
  • Entrez Gene: 12317 Mouse
  • Entrez Gene: 64202 Rat
  • Entrez Gene: 30248 Zebrafish
  • Omim: 109091 Human
  • SwissProt: P52193 Cow
  • SwissProt: P27797 Human

  • SwissProt: Q3TVD2 Mouse
  • SwissProt: P14211 Mouse
  • SwissProt: P18418 Rat
  • SwissProt: Q6PE26 Zebrafish
  • SwissProt: Q6NV30 Zebrafish
  • SwissProt: Q9PUC1 Zebrafish
  • Unigene: 515162 Human
  • Unigene: 1971 Mouse
  • Unigene: 467043 Mouse
  • Unigene: 974 Rat

  see all

• Alternative names
  Autoantigen RO antibodyAutoantigen RO antibodyCALR antibody

  CALR antibodyCALR protein antibodyCALR_HUMAN antibodyCalregulin antibodyCalreticulin antibodycC1qR antibodyCRP55 antibodyCRT antibodyCRTC antibodyEndoplasmic reticulum resident protein 60 antibodyERp60 antibodyFLJ26680 antibodygrp60 antibodyHACBP antibodyRO antibodySicca syndrome antigen A antibodySSA antibody

  see all

Anti-Calreticulin antibody - ER Marker images

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  Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab4)
  IF staining was performed on HCT15 colon cancer cells using a 1:50 dilution of the primary antibody followed by hybridisation with a 1:100 dilution of the secondary anti-rabbit antibody Alexa Fluor 488 (molecular Probes) with green fluorescence. Picture from review by Karin Birkenkamp-Demtroeder submitted 20 July 2004
  
• 

  Immunocytochemistry/ Immunofluorescence - Calreticulin antibody - ER Marker (ab4)
  ICC/IF image of ab4 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calreticulin antibody - ER Marker (ab4)
  ab4 staining calreticulin in Human placenta.
  Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Protocols

• Recommended WB protocol with ab4