| · Glycerol MSDS · Sodium Azide MSDS | |
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| Product Name | ADAM28 antibody - Aminoterminal end |
| | See all ADAM28 antibodies (3)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to ADAM28 - Aminoterminal end |
| Immunogen | Synthetic peptide based on the catalytic domain of human ADAM28.
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| Reacts with
(species key) | Hu |
| Specificity | ab40135 recognises the Aminoterminal region, catalytic domain of ADAM28. We have a range of domain specific antibodies for this target. For a full list please see all ADAM28 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 99.5 kDa, however, glycosylation and cysteine-rich regions make the reduced protein run at 114kDa in the unprocessed form and at 84 kDa in the processed form. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Cell Membrane and Secreted |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family |
| Relevance | ADAM28, also known as eMDCII, MDC-Ls and TECADAM, was first described in lymphocytes, also in Thymic Endothelial Cells (TECADAM), as well as in the epididymus. Later works describe two forms of ADAM28: ADAM28-S and ADAM28-L (short and long forms). The short form is a soluble, alternatively spliced form lacking the transmembrane and cytoplasmic domains. Message levels of the short form were found to be much lower than the long form in the tissues studied, but these may be tissue-specific differences. In humans, ADAM28 is reported in lymphocytes by some authors and to be absent from mature lymphocytes by others. The thyroid, lung, trachea, esophagus, and stomach have been shown to express human ADAM28 message. Knockout mice lacking B cells and T cells express ADAM28 message in the thyroid, lung, trachea, esophagus, and stomach. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM28 are still poorly understood. ADAM28 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM10, ADAM17) have been more thoroughly studied and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM28 (ADAM28 Long) sequence codes for a 775 amino acid protein with a predicted mass is 87.2 kDa, and a pI of 6.50. The soluble ADAM28 (ADAM-28 Short) is a 540 amino acid sequence, with a predicted mass of 61.3 kDa, and a pI of 6.58. Other forms of ADAM28 have been reported with slightly different carboxyterminal regions.
ADAM28 may play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. It may be involved in sperm maturation. There are 2 isoforms of ADAM28: one short secreted and one long membrane bound.
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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| Search PubMed (MEDLINE) for references to ADAM28 |
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