Overview
- Product nameAnti-MMP7 antibodySee all MMP7 primary antibodies ...
- DescriptionRabbit polyclonal to MMP7
- SpecificityThis antibody reacts with the 28 kDa protein (pro form) and 18 kDa (active form) of MMP 7.
- Tested applicationsWB, IHC-P, IHC-Fr, ICC, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A synthetic peptide from the human matrilysin (PUMP-1).
- Positive controlPlacenta.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab4044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: 1/50. |
| ICC | ICC: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
Target
- FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
- Sequence similaritiesBelongs to the peptidase M10A family.
- DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 4316 Human
- Entrez Gene: 4316 Human
- Entrez Gene: 4316 Human
- Entrez Gene: 17393 Mouse
- Omim: 178990 Human
- SwissProt: P09237 Human
- SwissProt: P09237 Human
- SwissProt: P09237 Human
- SwissProt: Q10738 Mouse
- Unigene: 2256 Human
- Unigene: 4825 Mouse
see all
Target information above from: UniProt accession
P09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Matrilysin antibodyMatrilysin uterine antibodyMatrin antibody
- Matrix Metalloproteinase 7 antibodyMatrix metalloproteinase-7 antibodyMMP 7 antibodyMMP-7 antibodyMMP7 antibodyMMP7_HUMAN antibodyMPSL1 antibodyPUMP 1 antibodyPump 1 protease antibodyPump-1 protease antibodyPUMP1 antibodyUterine Matrilysin antibodyUterine metalloproteinase antibody
see all
Anti-MMP7 antibody images
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Immunohistochemistry (Frozen sections) - MMP7 antibody (ab4044)This image is courtesy of an anonymous Abreviewab4044 staining MMP7 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/50 in blocking buffer for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP7 antibody (ab4044)This image is courtesy of an anonymous Abreviewab4044 at 1/50 dilution staining MMP7 in human stomach tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 1 hour at 23°C and then incubated with ab4044 for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit Ig was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - MMP7 antibody (ab4044)This image is courtesy of an anonymous Abreview.ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.
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Western blot - MMP7 antibody (ab4044)Anti-MMP7 antibody (ab4044) at 1/1000 dilution (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of Human AGS gastric carcinoma cells at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody (ab4044)Image from Stenvold H et al., Lung Cancer, Volume 75, Issue 2, February 2012, Pages 235–241. Fig 1.; doi: 10.1016/j.lungcan.2011.06.010. with permission from Elsevier.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-small-cell lung cancer tissue staining MMP7 with ab4044.
Antigen retrieval was performed by placing the sections in 0.01 M citrate buffer (pH 6) and heating in a microwave oven. Samples were incubated with primary antibody (1/15) overnight at 4°C. Staining was detected using an IHC substrate kit.
References for Anti-MMP7 antibody (ab4044)
This product has been referenced in:
- Phillips KL et al. Interleukin-1 receptor antagonist deficient mice provide insights into pathogenesis of human intervertebral disc degeneration. Ann Rheum Dis N/A:N/A (2013). IHC ; Mouse . Read more (PubMed: 23396662) »
- Hoeth M et al. The Transcription Factor SOX18 Regulates the Expression of Matrix Metalloproteinase 7 and Guidance Molecules in Human Endothelial Cells. PLoS One 7:e30982 (2012). IHC-P ; Human . Read more (PubMed: 22292085) »
