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Overview

  • Product nameSAA4 protein (His tag)See all SAA4 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 19-130 of Human SAA4 (with His-Tag at the N-terminus.)
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.002% Sodium Azide
    Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab40603 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS MS: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Amyloid A, serum, 4C SAAC-SAA
        Constitutively expressed serum amyloid A proteinCSAASAA 4SAA4SAA4 proteinSAA4_HUMANSerum amyloid A 4 proteinSerum amyloid A-4 proteinSerum amyloid A4 constitutive
      see all
  • FunctionMajor acute phase reactant. Apolipoprotein of the HDL complex.
  • Tissue specificityExpressed by the liver; secreted in plasma.
  • Sequence similaritiesBelongs to the SAA family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P35542 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SAA4 protein (His tag) images

    • SDS-PAGE - SAA4 peptide (19-130) (ab40603)
      SDS-PAGE - SAA4 peptide (19-130) (ab40603)
      Analysis of SAA4 Recombinant Protein (ab40603) @ 1µg using a 4-20% SDS gradient gel. Coomassie blue staining. A band is seen at about 17 kDa.
    • Western blot - SAA4 protein (His tag) (ab40603)
      Western blot - SAA4 protein (His tag) (ab40603)
      All lanes : Mouse anti-SAA4 polyclonal at 10 µg/ml

      Lane 1 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
      Lane 2 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
      Lane 3 : Recombinant A-SAA at 14 µg/ml
      Lane 4 : SAA4 protein (His tag) (ab40603) at 10 µg

      Secondary
      Goat anti-mouse IgG-HRP at 1/10000 dilution
      developed using the ECL technique

      Predicted band size : 16 kDa
      Observed band size : 16 kDa


      Exposure time : 2 minutes

      Image courtesy of an anonymous Abreview.

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    References for SAA4 protein (His tag) (ab40603)

    ab40603 has not yet been referenced specifically in any publications.

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    Western blot Abreview for SAA4 protein (His tag)

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    Application Western blot

    Review text: Product was used as a positive control for C-SAA western blots of human acute phase plasma samples.
    Sample: Human Tissue lysate - whole (whole human acute phase plasma)
    Loading amount: 14 µg
    Specification: whole human acute phase plasma
    Treatment: heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
    Gel Running Conditions: Reduced Denaturing (4-12% Bis-Tris)
    Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Other product details
    Concentration: 10 µg/ml
    Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS, Tween-20 (0.1%)

    Primary antibody (in addition to 'SAA4 protein (His tag)')
    Name: Non-Abcam antibody was used: anti -SAA4, Abnova, #6291
    Host species: Mouse
    Clonality: Polyclonal
    Dilution: 1/2000

    Secondary antibody
    Name: Non-Abcam antibody was used: Goat anti-mouse IgG-HRP (Dako P0447)
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/10000

    Detection
    Detection method: ECL+
    Exposure: 2 minute(s) and 0 second(s)
    Bands: Specific: 16 kDa Non-specific: none kDa
    Positive control: The product was used as the positive control in the immunoblotting experiment (visible at 16 kDa) C-SAA is also observed in human healthy control plasma and human acute phase plasma. In plasma two bands are visible for the unglycosylated (13kDa) and glycosylated (17kDa) form
    Negative control: recombinant A-SAA (MBL International, #JM-4324)

    Additional data
    PubMed ID: 20463814: View
    Additional Notes: Used PVDF membrane (Immobilon P, Millipore, #IPVH0010) and Kodak Biomax XAR film (Sigma-Aldrich, # F5513) for detection.
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    Submitted Mar 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"