Anti-Histone H1.2 antibody - ChIP Grade (ab4086)

Overview

• Product nameAnti-Histone H1.2 antibody - ChIP GradeSee all Histone H1.2 primary antibodies ...
• Description
  Rabbit polyclonal to Histone H1.2 - ChIP Grade
• SpecificityThis antibody has only been tested on bulk HeLa histones. We expect this antibody to be specific for Histone H1.2 - however we have not tested this specifically on different histone H1 variants.
• Tested applicationsChIP, WB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide derived from residues 1 - 100 of Human Histone H1.2.

  Read Abcam's proprietary immunogen policy

  .

• Positive controlHeLa Histone preparation
• General notes

  
  

  Histone H1.2 is one of five known h1 variants (known as H1.1/2/3/4/5 and/or H1.a/b/c/d/e). The H1 variants differ from each other in the amino acid sequence of their N-terminal regions.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Primary antibody notes Histone H1.2 is one of five known h1 variants (known as H1.1/2/3/4/5 and/or H1.a/b/c/d/e). The H1 variants differ from each other in the amino acid sequence of their N-terminal regions.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Histones
  • Variants

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

Applications

Target

• FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
• Sequence similaritiesBelongs to the histone H1/H5 family.
  Contains 1 H15 (linker histone H1/H5 globular) domain.
• Cellular localizationNucleus. Chromosome.

Target information above from: UniProt accession P16403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3006 Human
  • Entrez Gene: 3006 Human
  • Omim: 142710 Human
  • SwissProt: P16403 Human
  • SwissProt: P16403 Human
  • Unigene: 7644 Human

• Alternative names
  H1 histone family member 2 antibodyH1.a antibodyH12_HUMAN antibody

  H1F2 antibodyHIST1H1C antibodyHistone 1 H1c antibodyHistone cluster 1 H1c antibodyHistone H1.2 antibodyHistone H1d antibodyMGC3992 antibody

  see all

Anti-Histone H1.2 antibody - ChIP Grade images

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  Western blot - Histone H1.2 antibody - ChIP Grade (ab4086)
  
  
  Predicted band size : 21 kDa
  
  
  ab4086 Histone H1.2
  
  The antibody was used at a dilution of 1/500
  
  Lane 1: HeLa Histone (5ug) + ab4086
  Lane 2: HeLa Histone (5ug) + ab4086 + 1µg/ml of peptide (Histone H1.2) (ab16936)
  
  Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
  
  Exposure time: 1 minute
  Expected molecular weight: 21.3 kDa
• 

  Immunocytochemistry/ Immunofluorescence - Histone H1.2 antibody (ab4086)

  ICC/IF image of ab4086 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 10 min) and incubated with the antibody (ab4086, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

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  ChIP - Histone H1.2 antibody - ChIP Grade (ab4086)

  Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of  ab4086 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
      

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H1.2 antibody - ChIP Grade (ab4086)
  IHC image of Histone H1.2 staining in human breast carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4086, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.