· Glycerol MSDS
· Sodium Azide MSDS
| | Western blot - Kallikrein 4 antibody - Catalytic domain (ab40950)
(enlarge)
| |
|
| Product Name | Kallikrein 4 antibody - Catalytic domain |
| | See all Kallikrein 4 antibodies (4)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Kallikrein 4 - Catalytic domain |
| Immunogen | Synthetic peptide based on the catalytic domain of human Kallikrein 4.(Peptide available as ab43086.) |
| Reacts with
(species key) | Hu |
| Specificity | This antibody is specific for Kallikrein 4, it does not react with the other Kallikreins (1-3, 5-15). We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 4 antibodies |
| Tested applications
(see key) | SDS-PAGE, WB |
|
| Abreviews | |
|
| Application notes
(see key) | Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution. Extensive glycosylation and other posttranslational modifications give Kallikrein an apparent mass of 30-32 kDa on SDS PAGE gels. WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 27 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Secreted |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins |
| Relevance | The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer.
Kallikrein 4 (hK4) was first described as enamel matrix serine proteinase 1, a serine protease involved in degradation of amelogenin during tooth development and tooth enamel turnover. Unlike another enamel processing enzyme, MMP20, hK4 is not restricted to dental tissues and has been identified in a range of normal and cancerous tissues and cell lines. Androgen response elements have been identified in hK4 genes and the hK4 levels have been shown to be modified by androgens and estrogens, similar t |
my account
basket
help
contact us
Datasheet
References (0)
