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| | Western blot - Kallikrein 9 antibody - Catalytic domain (ab40955)
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| Product Name | Kallikrein 9 antibody - Catalytic domain |
| | See all Kallikrein 9 antibodies (3)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Kallikrein 9 - Catalytic domain |
| Immunogen | Synthetic peptide based on the catalytic domain of Human Kallikrein 9.
(Peptide available as ab43091.) |
| Reacts with
(species key) | Hu |
| Specificity | Ab40955 reacts with human Kallikrein 9 and does not react with the other human kallikreins (hK1-8, 10-15). We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 9 antibodies |
| Tested applications
(see key) | WB |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 36 kDa. Glycosylation and other post-translational modifications make the Kallikrein 9 protein run more slowly on SDS PAGE gels, usually around 36 kDa. A range of different sized bands can be seen for Kallikrein 9 in different tissues and in cell culture media, ranging from 16-30 kDa and likely representing different proteolytically processed forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Secreted |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins |
| Relevance | Kallikrein 9, also known as Kallikrein-Like 3 (KLK-L3), is a chymotrypsin-like serine proteinase. Kallikrein 9 was discovered as the locus for kallikreins on chromosome 19 was more fully mapped and found by similarity to the other tissue kallikreins. Kallikrein 9 has been found in the ovary, thymus, testis, prostate, skin, breast and neuronal tissues and is made by many cell lines in culture. Kallikrein 9 levels in breast cancer and uterine cancer patients have been reported to drop as the disease progresses, thus hK9 might be considered a favorable prognostic marker. Different splice variants of hK9 have been reported, although it is not yet known if they produce functional proteins. The full length Kallikrein 9 encodes for a 250 amino acid protein, with a predicted mass of 27.5 kDa and a pI of 7.53. The 234 amino acid form predicts a protein of 26 kDa with a pI of 9.76 and this quite basic pI might give the shorter form a very different function or localization. The shorter sequence also diverges before the catalytic serine residue, making it unlikely to be proteolytically active. Pre-pro-kallikrein 9 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein 9 is activated to Kallikrein 9 by removal of the 5 amino acid propeptide domain. |
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